Department of Food and Nutrition, School of Pharmaceutical Science, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil.
Medical, Scientific and Regulatory Affairs - Nestlé Nutrition/Nestlé Brazil Ltda, São Paulo, Brazil.
Food Res Int. 2021 May;143:110292. doi: 10.1016/j.foodres.2021.110292. Epub 2021 Mar 9.
Infant́s gut microbiota can be modulated by many factors, including mode of delivery, feeding regime, maternal diet/weight and probiotic and prebiotic consumption. The gut microbiota in dysbiosis has been associated with innumerous diseases. In this sense, early childhood intestinal microbiome modulation can be a strategy for disease prevention. This study had the purpose to evaluate the effect of an infant cereal with probiotic (Bifidobacterium animalis ssp. lactis BB-12®) on infant́s intestinal microbiota using SHIME®, which simulates human gastrointestinal conditions. The ascending colon was inoculated with fecal microbiota from three children (2-3 years old). NH, short chain fatty acids (SCFASs) and microbiota composition were determined by selective ion electrode, GC/MS and 16S sequencing, respectively. After treatment, butyric acid production increased (p < 0.05) 52% and a decrease in NH production was observed (p < 0.01). The treatment stimulated an increase (p < 0.01) of Lactobacillaceae families, more precisely L. gasseri and L. kefiri. L. gasseri has been associated with the prevention of allergic rhinitis in children and L. kefiri in the prevention of obesity. Thus, infant cereal with BB-12® is able to stimulate the growth of L. gasseri and L. kefiri in a beneficial way, reducing NH and increasing the production of SCFAs, especially butyric acid, in SHIME®.
婴儿的肠道微生物群可以通过许多因素来调节，包括分娩方式、喂养方式、母体饮食/体重以及益生菌和益生元的摄入。肠道微生物群失调与无数疾病有关。从这个意义上说，早期儿童肠道微生物群的调节可能是一种预防疾病的策略。本研究旨在使用模拟人体胃肠道条件的 SHIME®评估含有益生菌（动物双歧杆菌乳亚种 BB-12®）的婴儿谷物对婴儿肠道微生物群的影响。将来自三个儿童（2-3 岁）的粪便微生物群接种到升结肠中。通过选择性离子电极、GC/MS 和 16S 测序分别测定 NH、短链脂肪酸（SCFAs）和微生物群落组成。治疗后，丁酸产量增加（p<0.05）了 52%，同时 NH 的产量下降（p<0.01）。该治疗刺激了乳杆菌科家族（尤其是 L. gasseri 和 L. kefiri）的增加（p<0.01）。L. gasseri 与预防儿童过敏性鼻炎有关，而 L. kefiri 则与预防肥胖有关。因此，含有 BB-12®的婴儿谷物能够以有益的方式刺激 L. gasseri 和 L. kefiri 的生长，减少 NH 的产生，并增加 SHIME®中 SCFAs 的产生，特别是丁酸的产生。
