Koçer Canan, Benlier Necla, Balci Sibel Oğuzkan, Pehlivan Sacide, Şanli Maruf, Nacak Muradiye
Faculty of Medicine, Department of Medical Pharmacology, Gaziantep University, Gaziantep, Turkey.
Faculty of Medicine, Department of Medical Pharmacology, Sanko University, Gaziantep, Turkey.
Clin Respir J. 2020 Oct;14(10):948-955. doi: 10.1111/crj.13228. Epub 2020 Jul 20.
Lung cancer is a disease characterized by uncontrolled cell growth in the lung tissues. The most common causes of lung cancer include smoking, exposure to radon gas, asbestos, environmental pollutants as well as genetic factors. Nitric oxide (NO) has potential mutagenic and carcinogenic activity and may play an important role in lung cancer. Endothelial NO, synthesized from L-arginine by endothelial NO synthase (eNOS), inhibits apoptosis and promotes angiogenesis and tumor cell proliferation. The aim of the present study was to examine the possible relationship between eNOS gene intron 4 variable number of tandem repeat (VNTR) and exon 7-G894T (Glu298Asp) polymorphisms and lung cancer risk.
DNA was extracted from peripheral blood leukocytes of 107 lung cancer patients and 100 control subjects. Designated polymorphisms were identified by polymerase chain reaction (PCR) and/or restriction fragment length polymorphism (RFLP).
Our study showed that the frequencies of the b/b genotype and b allele of eNOS gene intron 4 VNTR polymorphism were significantly higher in lung cancer patients than in controls (P < 0.05). However, there was no significant association between eNOS gene G894T polymorphism and lung cancer risk (P > 0.05).
These results suggest that the presence of the intron 4 VNTR* b allele and b/b genotype may be a genetic risk factor for development of lung cancer. Further larger-scale studies are needed to confirm these findings.
肺癌是一种以肺组织细胞生长失控为特征的疾病。肺癌最常见的病因包括吸烟、接触氡气、石棉、环境污染物以及遗传因素。一氧化氮（NO）具有潜在的致突变和致癌活性，可能在肺癌中起重要作用。内皮型一氧化氮由内皮型一氧化氮合酶（eNOS）从L-精氨酸合成，可抑制细胞凋亡，促进血管生成和肿瘤细胞增殖。本研究的目的是探讨eNOS基因内含子4可变数目串联重复序列（VNTR）和外显子7-G894T（Glu298Asp）多态性与肺癌风险之间的可能关系。
从107例肺癌患者和100例对照者的外周血白细胞中提取DNA。通过聚合酶链反应（PCR）和/或限制性片段长度多态性（RFLP）鉴定指定的多态性。
我们的研究表明，肺癌患者中eNOS基因内含子4 VNTR多态性的b/b基因型和b等位基因频率显著高于对照组（P<0.05）。然而，eNOS基因G894T多态性与肺癌风险之间无显著关联（P>0.05）。
这些结果表明，内含子4 VNTR*b等位基因和b/b基因型的存在可能是肺癌发生的遗传危险因素。需要进一步的大规模研究来证实这些发现。
