Hautus M A, Kortbeek L M, Vetter J C, Laarman J J
Laboratory of Parasitology, University of Amsterdam, The Netherlands.
Trans R Soc Trop Med Hyg. 1988;82(6):858-61. doi: 10.1016/0035-9203(88)90019-3.
Six fresh Giardia lamblia strains were isolated by in vitro excystation and subsequent culturing of excysted parasites in bile-supplemented BI-S-33 medium. The cysts passed in faeces appeared to differ in structure when observed using differential interference contrast microscopy. Sometimes the enclosed organisms were closely applied to the cyst wall; in most stool specimens, however, the parasites were separated from the cyst wall by a space. Cysts with parasites closely applied to the wall were the most viable type, with excystation rates up to 90%. Cysts with organisms detached from the wall displayed low excystation rates, 0-15%. During exposure to the induction solution of hydrochloric acid (pH 2), parasites initially closely applied to the cyst walls completely detached, and after transfer into culture medium vigorous flexing movements of the organisms were observed. Stool samples from 42 symptomatic and asymptomatic giardiasis patients were examined; in 26 of the samples parasites hatched, and 6 new strains were established in axenic culture.
通过体外脱囊并随后在补充胆汁的BI-S-33培养基中培养脱囊后的寄生虫，分离出6株新鲜的蓝氏贾第鞭毛虫菌株。使用微分干涉相差显微镜观察时，粪便中排出的囊肿在结构上似乎有所不同。有时，包囊内的生物体紧密贴附于囊壁；然而，在大多数粪便样本中，寄生虫与囊壁之间存在间隙。寄生虫紧密贴附于壁的囊肿是最具活力的类型，脱囊率高达90%。生物体与壁分离的囊肿脱囊率较低，为0-15%。在暴露于盐酸诱导溶液（pH 2）期间，最初紧密贴附于囊壁的寄生虫完全分离，转移至培养基后观察到生物体剧烈的弯曲运动。对42例有症状和无症状贾第虫病患者的粪便样本进行了检查；其中26份样本中的寄生虫孵化出来，并在无菌培养中建立了6株新菌株。
