Department of Plant Pathology and Microbiology, National Taiwan University, Taipei 10617, Taiwan.
Genes (Basel). 2020 Jun 19;11(6):669. doi: 10.3390/genes11060669.
The heterothallic basidiomycetous fungus has two mating types, and α. Morphological progression of bisexual reproduction in is as follows: yeast to hyphal transition, filament extension, basidium formation, meiosis, and sporulation. Cdk-related kinase 1 () is a negative regulator of bisexual mating. In this study, we characterized the morphological features of mating structures in the mutant and determined the genetic interaction of in the regulatory networks of sexual differentiation. In the bilateral mutant cross, despite shorter length of filaments than in the wild-type cross, dikaryotic filaments and other structures still remained intact during bisexual mating, but the timing of basidium formation was approximately 18 h earlier than in the cross between wild type strains. Furthermore, gene expression analyses revealed that modulated the expression of genes involved in the progression of hyphal elongation, basidium formation, karyogamy and meiosis. Phenotypic results showed that, although deletion of gene increased the efficiency of bisexual mating, filamentation in the mutant was blocked by or mutation. A bioinformatics survey predicted the GATA transcriptional factor Gat1 as a potential substrate of Crk1 kinase. Our genetic and phenotypic findings revealed that and formed a regulatory circuit to negatively regulate to control filamentation progression and transition during bisexual mating.
异宗配合担子菌 有两种交配型， 和 α。 在 中两性生殖的形态发生进展如下：酵母到菌丝的转变、菌丝延伸、担子形成、减数分裂和孢子形成。细胞周期蛋白依赖性激酶相关激酶 1 () 是两性交配的负调控因子。在这项研究中，我们描述了 突变体中交配结构的形态特征，并确定了 在性分化调控网络中的遗传相互作用。在双侧 突变体杂交中，尽管与野生型杂交相比，二倍体菌丝的长度较短，但在两性交配过程中仍保持二倍体菌丝和其他结构的完整性，但是担子形成的时间比野生型菌株的杂交早约 18 小时。此外，基因表达分析表明， 调节了参与菌丝延伸、担子形成、核融合和减数分裂进程的基因的表达。表型结果表明，尽管 删除 基因增加了两性交配的效率，但 或 突变会阻断 突变体中的菌丝形成。生物信息学调查预测了 GATA 转录因子 Gat1 作为 Crk1 激酶的潜在底物。我们的遗传和表型发现表明， 和 形成了一个调节回路，以负调控 来控制两性交配过程中的菌丝延伸和过渡。
