Higuchi Yujiro
Department of Bioscience and Biotechnology; Faculty of Agriculture; Kyushu University , Hakozaki; Fukuoka, Japan.
Commun Integr Biol. 2015 Apr 29;8(2):e1025187. doi: 10.1080/19420889.2015.1025187. eCollection 2015 Mar-Apr.
Fungal plant pathogenicity is facilitated by effector proteins that are specifically expressed during infection and are responsible for suppressing plant defense mechanisms. Recent studies have elucidated the detailed molecular mechanisms of effector action throughout fungal infection. However, little is known about the trafficking and secretion of effectors in fungal hyphae during the initial stage of infection. Using state-of-the-art microscopy we have demonstrated that early endosome (EE) motility is required for effector production during fungal infection. Moreover, the MAPK Crk1 has been shown to travel on EEs and to function as a negative regulator of effector expression, suggesting that motile EEs are involved in signal transduction. Here I further discuss possible mechanisms whereby EE motility regulates effector expression in the initial stages of infection.
真菌植物致病性由效应蛋白促成，这些效应蛋白在感染过程中特异性表达，并负责抑制植物防御机制。最近的研究阐明了效应蛋白在整个真菌感染过程中的详细分子作用机制。然而，对于感染初期真菌菌丝中效应蛋白的运输和分泌了解甚少。利用最先进的显微镜技术，我们已经证明早期内体（EE）的运动是真菌感染过程中效应蛋白产生所必需的。此外，丝裂原活化蛋白激酶Crk1已被证明在早期内体上移动，并作为效应蛋白表达的负调节因子发挥作用，这表明移动的早期内体参与信号转导。在此，我将进一步讨论早期内体运动在感染初期调节效应蛋白表达的可能机制。
