Lee S W, Tsou A P, Chan H, Thomas J, Petrie K, Eugui E M, Allison A C
Department of Immunology, Syntex Research, Palo Alto, CA 94303.
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1204-8. doi: 10.1073/pnas.85.4.1204.
Transcription of the interleukin 1 beta (IL-1 beta) gene was studied by mRNA hybridization with a cDNA probe in the human promonocytic cell line U-937. Phorbol ester and lipopolysaccharide increased the steady-state level of IL-1 beta mRNA. Glucocorticoids markedly decreased IL-1 beta mRNA levels by two mechanisms. Transcription of the IL-1 gene was inhibited, as shown by in vitro transcription assays with nuclei isolated from glucocorticoid-treated cells. Moreover, kinetic analyses and pulse-labeling of mRNAs showed that glucocorticoids selectively decrease the stability of IL-1 beta mRNA, without affecting the stability of beta-actin and FOS mRNAs. Inhibition of the formation and effects IL-1 is a mechanism by which glucocorticoids can exert antiinflammatory and immunosuppressive effects.
通过与人早幼粒细胞系U - 937中的cDNA探针进行mRNA杂交，研究了白细胞介素1β（IL - 1β）基因的转录。佛波酯和脂多糖增加了IL - 1β mRNA的稳态水平。糖皮质激素通过两种机制显著降低IL - 1β mRNA水平。用从糖皮质激素处理的细胞中分离的细胞核进行的体外转录分析表明，IL - 1基因的转录受到抑制。此外，mRNA的动力学分析和脉冲标记表明，糖皮质激素选择性地降低IL - 1β mRNA的稳定性，而不影响β - 肌动蛋白和FOS mRNA的稳定性。抑制IL - 1的形成及其作用是糖皮质激素发挥抗炎和免疫抑制作用的一种机制。
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