Appliances Company, Panasonic Corporation, 3-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-kitamachi, Musashino City, Tokyo 180-8633, Japan.
Biointerphases. 2020 Jun 23;15(3):031013. doi: 10.1116/6.0000017.
In this study, the authors evaluated the distribution of low-abundance beauty ingredients in human skin tissues. The distribution of collagen tripeptide, a beauty ingredient, in the human skin was evaluated by applying multivariate curve resolution (MCR) to the time-of-flight secondary ion mass spectrometry mapping data, including reference information. The intensity of the secondary ion peaks was increased by the accumulation of secondary ion intensity in the depth direction obtained by argon cluster sputtering. Consequently, the collagen tripeptide distribution in the skin was evaluated by separating it from collagen peptide, although the conventional analysis was difficult because of the dilution of the collagen tripeptide in the skin. Additionally, the distribution of the collagen tripeptide in the skin may be determined with an autoencoder. In this study, the distribution of small amounts of beauty ingredients (the collagen tripeptide) in the skin was observed by integrating the secondary ion intensity in the depth direction with Ar cluster sputtering and applying MCR or autoencoder.
在这项研究中，作者评估了低丰度美容成分在人体皮肤组织中的分布。通过将飞行时间二次离子质谱成像数据（包括参考信息）应用于多元曲线分辨（MCR），评估了美容成分胶原三肽在人体皮肤中的分布。通过氩簇溅射获得的深度方向上的二次离子强度的累积，增加了二次离子峰的强度。因此，尽管由于胶原三肽在皮肤中的稀释，常规分析比较困难，但仍可以通过分离胶原肽来评估皮肤中的胶原三肽分布。此外，还可以通过自动编码器来确定胶原三肽在皮肤中的分布。在这项研究中，通过将深度方向上的二次离子强度与 Ar 簇溅射相结合，并应用 MCR 或自动编码器，观察到了皮肤中少量美容成分（胶原三肽）的分布。
