Trejos-Delgado Catalina, Cadavid-Restrepo Gloria E, Hormaza-Anaguano Angelina, Agudelo Edison A, Barrios-Ziolo Leonardo, Loaiza-Usuga Juan Carlos, Cardona-Gallo Santiago A
Universidad Nacional de Colombia, Campus Medellin, Mine Faculty, Geosciences and Environment Department, Bioremediation and Technological Development Group, Carrera 65 N.º 59ª- 110, Medellín, Colombia.
Universidad Nacional de Colombia, Campus Medellin, Sciences Faculty, SIRYTCOR (Synthesis, Reactivity and Organic Compounds Transformation Group), Microbiodiversity and Bioprospection Group, Cellular Biology and Molecular Laboratory, Carrera 65 N.º 59ª- 110, Medellín, Colombia.
An Acad Bras Cienc. 2020 Jun 22;92(2):e20181396. doi: 10.1590/0001-3765202020181396. eCollection 2020.
This research was implemented in the Colombian Amazon forest area; to assess the effect of Tween-80® surfactant in the degradation of the Total Petroleum Hydrocarbons (TPH) in bioremediation treatments under aerobic conditions in the laboratory and pilot-scale. One control treatment, Natural Attenuation (AT) and four biostimulation treatments with leonardite with four different dosages of Tween-80® were proposed. The efficacy of organic stimulators and nonionic surfactant in soil microbiota was studied at laboratory and pilot scales, the latter in a passive aeration reactor. The test that presented a better performance was carried out with a Convective Flow Reactor (CFR) at pilot-scale. The results showed that bioremediation strategies improved the natural degradation process; the best outcomes were obtained in a treatment that includes Leonardite and Tween-80® (1.5 g/L) with 52% TPH degradation in 80 days (d).Tween-80® produced an effect in TPH solubility, and increased the production of CO2 in distinctive bioremediation treatments in both periods. The kinetics of CO2 production showed that the system required a periodic addition of a co-substrate as well as an increase of soil microbiota through the addition of compost (pilot scale). In this stage more than 76% of contaminant was degraded in 60d.
本研究在哥伦比亚亚马逊森林地区开展；旨在评估吐温80®表面活性剂在实验室和好氧条件下的中试规模生物修复处理中对总石油烃（TPH）降解的影响。提出了一种对照处理，即自然衰减（AT），以及四种添加不同剂量吐温80®的风化煤生物刺激处理。在实验室和中试规模下研究了有机刺激剂和非离子表面活性剂对土壤微生物群的功效，后者在一个被动曝气反应器中进行。在中试规模下，使用对流流动反应器（CFR）进行了表现最佳的试验。结果表明，生物修复策略改善了自然降解过程；在一种包含风化煤和吐温80®（1.5 g/L）的处理中取得了最佳结果，80天内TPH降解率达到52%。吐温80®对TPH溶解度产生了影响，并在两个阶段的不同生物修复处理中增加了二氧化碳的产生。二氧化碳产生动力学表明，该系统需要定期添加共底物，并通过添加堆肥（中试规模）增加土壤微生物群。在这个阶段，60天内超过76%的污染物被降解。
