Tanaka Takahiro, Nishii Yuichi, Matsuo Hirotoshi, Takahashi Taku
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Plants (Basel). 2020 Jun 22;9(6):779. doi: 10.3390/plants9060779.
Map-based gene cloning has played a key role in many genetic studies using the model plant, . In the post- next generation sequencing era, identification of point mutations and their corresponding genes is increasingly becoming a powerful and important approach to define plant gene function. To perform initial mapping experiments efficiently on mutants, enrichment of easy-to-use and reliable polymorphic DNA markers would be desirable. We present here a list of InDel polymorphic markers between Col-0 and L-0 accessions that can be detected in standard agarose gel electrophoresis.
基于图谱的基因克隆在许多以模式植物进行的遗传研究中发挥了关键作用。在下一代测序时代之后，点突变及其相应基因的鉴定日益成为定义植物基因功能的一种强大且重要的方法。为了在突变体上高效地进行初步定位实验，富集易于使用且可靠的多态性DNA标记将是很有必要的。我们在此列出了在标准琼脂糖凝胶电泳中可检测到的Col-0和L-0种质之间的插入缺失多态性标记列表。
