Tsukamoto A, Kimura K, Ishii Y, Takano T, Yamane K
Institute of Biological Sciences, University of Tsukuba, Japan.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):25-31. doi: 10.1016/0006-291x(88)90554-2.
The nucleotide sequence of the gene for maltohexaose-producing amylase from an alkalophilic Bacillus sp. #707 was determined. Starting at an ATG initiation codon, an open reading frame was composed of 1554 bp (518 amino acids). The NH2-terminal portion encoded a 33 amino acid-long signal peptide. The deduced amino acid sequence of the extracellular mature enzyme was more than 60% homologous to those of the liquefying type alpha-amylases but not to those of the saccharifying type alpha-amylases. The sequence of its signal peptide was completely different from those of other alpha-amylases.
测定了嗜碱芽孢杆菌#707中产生麦芽六糖淀粉酶基因的核苷酸序列。从ATG起始密码子开始，一个开放阅读框由1554个碱基对（518个氨基酸）组成。NH2末端部分编码一个33个氨基酸长的信号肽。推导的细胞外成熟酶氨基酸序列与液化型α-淀粉酶的序列同源性超过60%，但与糖化型α-淀粉酶的序列不同源。其信号肽序列与其他α-淀粉酶的序列完全不同。
