Farag Azza Gaber Antar, El Askary Shymaa A, Fathy Waleed M, Elbassal Fathia, Azzam Ayman Ali, Tayel Nermin Reda, Abdul Karim Samah Saad, Shehata Wafaa Ahmed
Dermatology, Andrology & STDs Department, Faculty of Medicine, Menoufia University, Al Menoufia, Egypt.
Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Minufya, Egypt.
Clin Cosmet Investig Dermatol. 2020 Jun 2;13:379-389. doi: 10.2147/CCID.S240420. eCollection 2020.
Leprosy is a chronic contagious disease caused by . CD163 is a monocyte trans-membrane glycoprotein receptor (mCD163) that sheds from the cell surface and circulates as a soluble (serum) form (sCD163). Changes in the mCD163 and sCD163 levels could mirror the categorization of inflammatory procedure, demonstrating a possible use of CD163 as a diagnostic indicator of inflammation.
To investigate the possible role of CD163 (sCD163 and mCD163) in leprosy pathogenesis and to assess whether CD163 is a helpful inflammatory marker of leprosy development and typing.
This case control study included 70 leprosy patients and 30 healthy controls. Leprosy patients were classified according to the Madrid criteria (1953) into: tuberculoid leprosy (TT), border-line leprosy (BL), and lepromatous leprosy (LL). For all participants, complete blood count (CBC), serum CD163 using ELISA and monocytes positive for CD163 using flow cytometry were done.
Leprosy patients had significantly low WBCs and platelet counts (p<0.001) and had significantly higher sCD163 (p=0.025) and mCD163 (p=0.042) that were highest in LL followed by BL, then TT patients (p<0.001). There was a significant positive correlation between mCD163 and sCD163 levels in leprosy patients (r=0.896, p<0.001). ROC analysis revealed a significant role of serum sCD163 and of mCD163 positive monocytes in the detection (p<0.001) and typing of leprosy (p=0.002 and p<0.001, respectively).
Both sCD163 and mCD163 positive monocytes may have an active role in leprosy pathogenesis. They could be potential biomarkers for leprosy detection and typing.
麻风病是一种由……引起的慢性传染病。CD163是一种单核细胞跨膜糖蛋白受体（mCD163），它从细胞表面脱落并以可溶性（血清）形式（sCD163）循环。mCD163和sCD163水平的变化可以反映炎症过程的分类，表明CD163可能作为炎症的诊断指标。
研究CD163（sCD163和mCD163）在麻风病发病机制中的可能作用，并评估CD163是否是麻风病发展和分型的有用炎症标志物。
本病例对照研究包括70例麻风病患者和30例健康对照。麻风病患者根据马德里标准（1953年）分为：结核样型麻风（TT）、界线类麻风（BL）和瘤型麻风（LL）。对所有参与者进行全血细胞计数（CBC）、使用酶联免疫吸附测定法检测血清CD163以及使用流式细胞术检测CD163阳性单核细胞。
麻风病患者的白细胞和血小板计数显著降低（p<0.001），sCD163（p=0.025）和mCD163（p=0.042）显著升高，其中LL患者最高，其次是BL患者，然后是TT患者（p<0.001）。麻风病患者中mCD163和sCD163水平之间存在显著正相关（r=0.896，p<0.001）。ROC分析显示血清sCD163和mCD163阳性单核细胞在麻风病检测（p<0.001）和分型（分别为p=0.002和p<0.001）中具有显著作用。
sCD163和mCD163阳性单核细胞在麻风病发病机制中可能都发挥着积极作用。它们可能是麻风病检测和分型的潜在生物标志物。
