Gonçalves Carolyne Caetano, Bruce Thiago, Silva Caio de Oliveira Gorgulho, Fillho Edivaldo Ximenes Ferreira, Noronha Eliane Ferreira, Carlquist Magnus, Parachin Nádia Skorupa
Department of Genomic Science and Biotechnology, Universidade Católica de Brasília - UCB, Brasília, Brazil.
Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, Brazil.
Front Microbiol. 2020 Jun 9;11:1081. doi: 10.3389/fmicb.2020.01081. eCollection 2020.
Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid screening methods are crucial for time- and resource-efficient development of novel microbial strains and enzymes with applications in the lignin biorefinery. The present review gives an overview of recent developments and applications of a vast arsenal of activity and sequence-based methodologies for uncovering novel microbial strains with ligninolytic potential, novel enzymes for lignin depolymerization and for unraveling the main metabolic routes during growth on lignin. Finally, perspectives on the use of each of the presented methods and their respective advantages and disadvantages are discussed.
木质素是一种丰富的细胞壁成分，主要用于产生蒸汽和电力。然而，木质素的增值，即将木质素转化为高附加值的燃料、化学品或材料，对于全面实施具有成本效益的木质纤维素生物精炼厂至关重要。从这个角度来看，快速筛选方法对于高效开发具有木质素生物精炼应用潜力的新型微生物菌株和酶至关重要。本综述概述了大量基于活性和序列的方法的最新进展和应用，这些方法用于发现具有木质素分解潜力的新型微生物菌株、用于木质素解聚的新型酶以及揭示在木质素上生长期间的主要代谢途径。最后，讨论了所介绍的每种方法的使用及其各自优缺点的观点。
