Dortet Laurent, Bonnin Rémy A, Le Hello Simon, Fabre Laetitia, Bonnet Richard, Kostrzewa Markus, Filloux Alain, Larrouy-Maumus Gerald
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United Kingdom.
Department of Bacteriology-Hygiene, Bicêtre Hospital, Assistance Publique - Hôpitaux de Paris, Le Kremlin-Bicêtre, France.
Front Microbiol. 2020 Jun 3;11:1141. doi: 10.3389/fmicb.2020.01141. eCollection 2020.
Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.
大多数革兰氏阴性菌对多粘菌素的耐药性源于其脂多糖（LPS）脂质A部分的化学修饰，这是由染色体编码的突变或最近发现的质粒编码基因介导的，这些基因进一步使黏菌素耐药情况变得复杂。目前，通过肉汤微量稀释法测定最低抑菌浓度（MIC）是检测多粘菌素耐药性的金标准，但耗时（24小时），在临床和兽医实验室中操作具有挑战性。在此，我们展示了在常规基质辅助激光解吸电离（MALDI）Biotyper Sirius系统上使用MALDIxin检测黏菌素耐药性的方法。
