Department of Clinical Laboratory, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China.
Front Cell Infect Microbiol. 2022 Feb 11;12:761328. doi: 10.3389/fcimb.2022.761328. eCollection 2022.
The ability of VITEK mass spectrometry (MS) in detection of bacterial resistance is currently under exploration and evaluation. In this study, we developed and validated a VITEK MS method to rapidly test carbapenemase-producing (CPKP). Solvents, antibiotic concentrations, crystal conditions and times, centrifugation speeds, and other factors were optimized to design a rapid sample pretreatment process for CPKP detection by VITEK MS. The related parameters of the mass spectrum were adjusted on the instrument to establish an CPKP detection mode. 133 clinically isolated strains of CPKP in the microbiology laboratory at the Shenzhen People's Hospital from 2004 to 2017 were selected for accuracy evaluation. The fresh suspected strains from the microbiology laboratory in 2020 were used to complete the clinical verification. Two antibiotics, meropenem (MEM) and imipenem (IPM), were used as substrates. These two substrates were incubated with suspected CPKP, and the results were obtained by VITEK MS detection. Using this method, different types of CPKP showed different detection results and all the CPKP strains producing KPC-2 and IMP-4 carbapenemase were detected by VITEK MS. Thus, VITEK MS can be used for rapid detection of CPKP, especially for some common types of CPKP. This method provides high accuracy and speed of detection. Combined with its cost advantages, it can be intensely valuable in clinical microbiology laboratories after the standard operating procedures are determined.
VITEK 质谱（MS）在检测细菌耐药性方面的能力目前正在探索和评估中。在这项研究中，我们开发并验证了一种 VITEK MS 方法，用于快速检测产碳青霉烯酶的 （CPKP）。优化了溶剂、抗生素浓度、晶体条件和时间、离心速度等因素，以设计一种快速的 VITEK MS 检测 CPKP 的样品预处理方法。调整仪器上的质谱相关参数，建立 CPKP 检测模式。选择 2004 年至 2017 年深圳市人民医院微生物实验室分离的 133 株临床分离的 CPKP 菌株进行准确性评估。2020 年从微生物实验室采集新鲜疑似菌株进行临床验证。使用两种抗生素，美罗培南（MEM）和亚胺培南（IPM）作为底物。将这两种底物与疑似 CPKP 孵育，通过 VITEK MS 检测获得结果。使用该方法，不同类型的 CPKP 显示出不同的检测结果，所有产 KPC-2 和 IMP-4 碳青霉烯酶的 CPKP 菌株均通过 VITEK MS 检测到。因此，VITEK MS 可用于快速检测 CPKP，尤其是一些常见类型的 CPKP。该方法具有较高的检测准确性和速度。结合其成本优势，在确定标准操作程序后，它在临床微生物学实验室中具有很高的价值。
