Chu Guangyu, Miao Yingying, Huang Kexin, Song Han, Liu Liang
Department of Radiology, The Second Hospital of Jilin University, Changchun, China.
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, China.
Front Bioeng Biotechnol. 2020 Jun 9;8:509. doi: 10.3389/fbioe.2020.00509. eCollection 2020.
This work is to study the effect of Rhizopus nigrum polysaccharide EPS1-1 on hepatocellular carcinoma (HCC) and . HepG2 and Huh-7 cells and nude mice models of liver cancers were used in this study. The cells and nude mice were treated with EPS1-1 at different concentrations. The CCK8 assays were used to measure the proliferation activities of cells, apoptosis was determined with flow cytometry, cell migration was measured by wound-healing assays, cell invasion was evaluated by Transwell assay, and the survival periods of different groups of tumor-bearing mice were compared. Real-time PCR and Western blot were used to measure the expression levels of mRNAs and proteins of the genes related to proliferation, apoptosis, migration, and invasion. experiments revealed that when treated with EPS1-1, HepG2 and Huh-7 cell proliferation activities decreased, while there was an increase for the apoptosis rate, and the migration and invasion capabilities were significantly reduced. experiments showed that EPS1-1 could significantly reduce the tumor growth and lung metastasis of HCC, and prolong the survival periods of tumor-bearing nude mice. Furthermore, EPS1-1 has no apparent damage to the heart, liver, and kidney. Further studies showed that EPS1-1 could affect the expression of proliferation-related genes CCND1 and c-Myc, apoptosis-related genes BAX and Bcl-2, and migration and invasion related genes Vimentin and Slug, thereby affecting the biological process of HCC. EPS1-1 can inhibit the malignant process of HCC and , which indicates that EPS1-1 has the potential value of clinical application as chemotherapy or adjuvant in the treatment of liver cancer.
本研究旨在探讨黑根霉多糖EPS1-1对肝细胞癌（HCC）的影响。本研究使用了HepG2和Huh-7细胞以及肝癌裸鼠模型。细胞和裸鼠分别用不同浓度的EPS1-1进行处理。采用CCK8法检测细胞增殖活性，通过流式细胞术检测细胞凋亡，采用伤口愈合试验检测细胞迁移，通过Transwell试验评估细胞侵袭，并比较不同组荷瘤小鼠的生存期。采用实时荧光定量PCR和蛋白质免疫印迹法检测与增殖、凋亡、迁移和侵袭相关基因的mRNA和蛋白质表达水平。实验结果显示，用EPS1-1处理后，HepG2和Huh-7细胞的增殖活性降低，而凋亡率升高，迁移和侵袭能力显著降低。实验表明，EPS1-1可显著抑制HCC的肿瘤生长和肺转移，并延长荷瘤裸鼠的生存期。此外，EPS1-1对心脏、肝脏和肾脏无明显损伤。进一步研究表明，EPS1-1可影响增殖相关基因CCND1和c-Myc、凋亡相关基因BAX和Bcl-2以及迁移和侵袭相关基因波形蛋白（Vimentin）和锌指蛋白Slug的表达，从而影响HCC的生物学进程。EPS1-1可抑制HCC的恶性进程，这表明EPS1-1作为化疗药物或辅助药物在肝癌治疗中具有潜在的临床应用价值。
