School of Life Science, Shandong University, Jinan 250100, PR China.
Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241002, PR China.
Biomed Pharmacother. 2018 Jul;103:738-745. doi: 10.1016/j.biopha.2018.04.093. Epub 2018 Apr 24.
Polysaccharide (EPS1-1) extracted from fermentation liquor of Rhizopus nigricans possesses antitumor and immune-enhancing activities. The study was the first to investigate the anti-metastasis effects of EPS1-1 in vitro and in vivo. Results suggested that EPS1-1 dose-dependently suppressed the migration, invasion and adhesion abilities of CT26 cells. Furthermore, EPS1-1 dramatically inhibited the enzyme activity and expression levels of matrix metalloproteinases (MMPs) in CT26 cells, as well as the tube formation of HUVECs. Similar results were observed in the lung metastasis mice which were administrated with EPS1-1 for 14 d. EPS1-1 could inhibit angiogenesis by decreasing the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in lung tissue. Moreover, vimentin, as a marker molecule in epithelial-mesenchymal transition (EMT) which is closely related to metastasis, was found to be down expression by EPS1-1 in CT26 cells and lung tissue. These results suggested that EPS1-1 could suppress metastasis in vitro and in vivo by inhibiting invasion and angiogenesis, which provides potential application to against colorectal cancer metastasis.
从黑根霉发酵液中提取的多糖（EPS1-1）具有抗肿瘤和免疫增强活性。这项研究首次探讨了 EPS1-1 在体外和体内的抗转移作用。结果表明，EPS1-1 可剂量依赖性地抑制 CT26 细胞的迁移、侵袭和黏附能力。此外，EPS1-1 还显著抑制 CT26 细胞中基质金属蛋白酶（MMPs）的酶活性和表达水平，以及 HUVECs 的管形成。在给予 EPS1-1 治疗 14 天的肺转移小鼠中也观察到了类似的结果。EPS1-1 可通过降低肺组织中血管内皮生长因子（VEGF）和微血管密度（MVD）的表达来抑制血管生成。此外，波形蛋白作为上皮-间充质转化（EMT）的标志物分子，与转移密切相关，发现在 CT26 细胞和肺组织中，EPS1-1 可下调其表达。这些结果表明，EPS1-1 可通过抑制侵袭和血管生成来抑制体内外的转移，为防治结直肠癌转移提供了潜在的应用价值。
