Abe T, Nawa Y
Department of Parasitology, Miyazaki Medical College, Japan.
Immunology. 1988 Feb;63(2):181-5.
After a primary infection of congenitally athymic nu/nu mice with Strongyloides ratti, worms were not expelled and the number of intestinal mucosal mast cells (MMC) remained at a low level. When S. ratti-infected nu/nu mice were treated by repetitive injections of semi-purified IL-3 from Day 5 to Day 10 post-infection (total 1.4 X 10(5) U), significant reduction of larval excretion in faeces (LPG) was observed on Day 13. The number of adult worms in the small intestine of IL-3-treated mice was significantly lower than that of untreated, infected nude mice. The higher dose of IL-3 treatment from Day 4 to Day 13 (total 5.8 X 10(5) U) caused more profound reduction of LPG as early as Day 9, although complete cessation of LPG was not observed until Day 13, the end of this series of experiments. By this higher dose of IL-3 treatment, adult worms were completely expelled from the small intestine, although a small number of residual worms, which could explain the persistent low level of LPG detected from Day 9 to Day 13, was found in the caecum. Histological examination revealed that the number of MMC, especially in the epithelium, of the small intestine of IL-3-treated mice was significantly higher than that of untreated mice.
先天性无胸腺裸鼠经大鼠类圆线虫初次感染后，蠕虫未被排出，肠道黏膜肥大细胞（MMC）数量维持在低水平。当感染大鼠类圆线虫的裸鼠在感染后第5天至第10天通过重复注射半纯化的白细胞介素-3进行治疗（总量为1.4×10⁵单位）时，在第13天观察到粪便中幼虫排泄量（LPG）显著减少。白细胞介素-3治疗组小鼠小肠内成虫数量显著低于未治疗的感染裸鼠。从第4天至第13天用更高剂量的白细胞介素-3治疗（总量为5.8×10⁵单位），早在第9天就使LPG有更显著的减少，尽管直到本系列实验结束的第13天LPG才完全停止排出。通过这种更高剂量的白细胞介素-3治疗，成虫从小肠中被完全排出，尽管在盲肠中发现了少量残留蠕虫，这可以解释从第9天至第13天检测到的LPG持续处于低水平的现象。组织学检查显示，白细胞介素-3治疗组小鼠小肠，尤其是上皮中的MMC数量显著高于未治疗小鼠。
