Shaw J, Meerovitch K, Bleackley R C, Paetkau V
Department of Biochemistry, University of Alberta, Edmonton, Canada.
J Immunol. 1988 Apr 1;140(7):2243-8.
IL-2 mRNA has a t1/2 of 1 to 2 h in T lymphocyte cell lines and in activated human PBL. Human Jurkat cells show a rapid increase of IL-2 mRNA on induction of IL-2 synthesis, followed by an equally rapid decline 4 to 6 h later. The decline occurs despite a high rate of synthesis, and appears to be due to an enhanced rate of IL-2 mRNA degradation. The degradation of IL-2 mRNA is selectively sensitive to cycloheximide and actinomycin D, inhibitors of protein and RNA synthesis, respectively. IL-2 mRNA levels in mouse EL4.E1 T lymphoma cells, and in activated human PBL, decline rapidly on removal of the inducing agents, indicating that transcription continues only as long as the activating signal is present. The transience of IL-2 mRNA is seen as an important property of a transiently acting immunoregulatory factor.
在T淋巴细胞系和活化的人外周血淋巴细胞中，白细胞介素-2（IL-2）信使核糖核酸（mRNA）的半衰期为1至2小时。人Jurkat细胞在诱导IL-2合成时，IL-2 mRNA迅速增加，随后在4至6小时后同样迅速下降。尽管合成速率很高，但仍会出现下降，这似乎是由于IL-2 mRNA降解速率加快所致。IL-2 mRNA的降解分别对蛋白质合成抑制剂环己酰亚胺和RNA合成抑制剂放线菌素D敏感。在去除诱导剂后，小鼠EL4.E1 T淋巴瘤细胞和活化的人外周血淋巴细胞中的IL-2 mRNA水平迅速下降，这表明只有在激活信号存在时转录才会继续。IL-2 mRNA的短暂性被视为一种瞬时作用的免疫调节因子的重要特性。
