凋亡相关基因和 microRNAs 的失调表达及其在胃癌发生中的作用。
Dysregulated Expression of Apoptosis-Associated Genes and MicroRNAs and Their Involvement in Gastric Carcinogenesis.
机构信息
Universidade do Sagrado Coração (USC), Bauru, São Paulo, Brazil.
Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
出版信息
J Gastrointest Cancer. 2021 Jun;52(2):625-633. doi: 10.1007/s12029-019-00353-3.
PURPOSE
Analyze the expression of caspase-9, Smac/DIABLO, XIAP, let-7a, and let-7b in patients with normal gastric tissue, chronic gastritis, and gastric adenocarcinoma.
METHODS
The expression of caspase-9, Smac/DIABLO, XIAP, let-7a, and let-7b by qRT-PCR was analyzed in 158 samples from 53 patients with normal gastric mucosa, 86 with chronic gastritis, and 19 with gastric cancer.
RESULTS
The comparison between the gastric cancer and the control group revealed a decreased expression of caspase-9 in gastric cancer tissues; considering the Helicobacter pylor presence, comparable results were revealed. Smac/DIABLO was increased in gastric cancer cells, while XIAP demonstrated no significant difference in the gene expression. The microRNA analysis revealed a decreased expression of let-7a and let-7b in samples positive to H. pylori infection and in gastric cancer group, regardless of the presence of the bacterium.
CONCLUSION
Our study provided some evidence of low activity of the intrinsic apoptosis pathway, as well as the influence of H. pylori on let-7a and let-7b expression.
目的
分析 caspase-9、Smac/DIABLO、XIAP、let-7a 和 let-7b 在正常胃组织、慢性萎缩性胃炎和胃腺癌患者中的表达。
方法
采用 qRT-PCR 法分析 53 例正常胃黏膜、86 例慢性萎缩性胃炎和 19 例胃癌患者 158 例样本中 caspase-9、Smac/DIABLO、XIAP、let-7a 和 let-7b 的表达。
结果
与对照组相比，胃癌组织中 caspase-9 的表达降低；考虑到幽门螺杆菌的存在，结果相似。Smac/DIABLO 在胃癌细胞中增加，而 XIAP 在基因表达上没有显著差异。miRNA 分析显示，无论是否存在细菌，感染幽门螺杆菌的样本和胃癌组中 let-7a 和 let-7b 的表达均降低。
结论
本研究提供了一些证据表明内在凋亡途径活性降低，以及幽门螺杆菌对 let-7a 和 let-7b 表达的影响。
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