胃黏膜炎症和胃癌中 miR-125a-5p 的减少及其与 H. pylori 的可能关联。
Decrease of miR-125a-5p in Gastritis and Gastric Cancer and Its Possible Association with H. pylori.
机构信息
Genetics Laboratory, Marília Medical School (FAMEMA), Lourival Freire, 240, Bairro Fragata, Marília, São Paulo, CEP 17519-050, Brazil.
Institute of Tropical Pathology and Public Health, Federal University of Goiás (UFG), Goiânia, Goiás, Brazil.
出版信息
J Gastrointest Cancer. 2021 Jun;52(2):569-574. doi: 10.1007/s12029-020-00432-w.
PURPOSE
The aim of this study was to evaluate the expression of miR-125a-5p in patients with dyspeptic symptoms and gastric cancer, correlating them with the development of this cancer and H. pylori.
METHODS
Patients were divided in groups according to histopathological analysis (control, gastritis, and cancer groups). Polymerase chain reaction was performed to detect H. pylori and real-time quantitative PCR to determine miR-125a-5p expression.
RESULTS
H. pylori was detected in 44% of the patients, with prevalence in the gastritis and cancer groups. A statistically significant decrease of miR-125a-5p expression was found in the control positive (p = 0.0183*), gastritis positive (p = 0.0380*), and cancer positive (p = 0.0288*) groups when compared with the control negative group.
CONCLUSION
We suggest that decreased expression of the miRNA-125a-5p associated with the presence of the H. pylori is an important mechanism in gastric diseases and could be a possible marker for early diagnosis of gastric cancer.
目的
本研究旨在评估 miR-125a-5p 在消化不良症状和胃癌患者中的表达，并与这种癌症和 H. pylori 的发生相关联。
方法
根据组织病理学分析（对照组、胃炎组和癌症组）将患者分组。聚合酶链反应用于检测 H. pylori，实时定量 PCR 用于确定 miR-125a-5p 的表达。
结果
在 44%的患者中检测到 H. pylori，在胃炎和癌症组中更为普遍。与对照组阴性组相比，对照组阳性组（p=0.0183*）、胃炎阳性组（p=0.0380*）和癌症阳性组（p=0.0288*）中 miR-125a-5p 的表达明显降低。
结论
我们认为与 H. pylori 存在相关的 miRNA-125a-5p 表达降低是胃部疾病的一个重要机制，可能是胃癌早期诊断的一个潜在标志物。
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