NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.
Institute of Obstetrics and Gynecology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, China.
Am J Reprod Immunol. 2020 Oct;84(4):e13295. doi: 10.1111/aji.13295. Epub 2020 Jul 11.
Endometrial hyperplasia (EH) is characterized by an endometrial gland-to-stroma ratio >1 and is one of the most common gynecological diseases in the world. The role of immunocyte subsets in the development of EH remains unknown.
Patients who underwent dilatation and curettage due to abnormal uterine bleeding were recruited in the present study. Alterations in the numbers of different types of immune cell subsets in the endometrium of patients were analyzed by flow cytometry.
The present study included 48 patients who were divided into three groups, based on the pathological results: (a) proliferative period (PP, n = 12); (b) simple EH (SEH, n = 30); and (c) complex EH (CEH, n = 6). The results showed that immune cell subpopulations were significantly different between these three groups. Compared with the PP group, the proportion of CD45 cells and neutrophils and the subtypes of T cells and macrophages were significantly increased in the SEH patients. Compared with the PP and SEH groups, subsets of immunocytes in the CEH group were significantly decreased, including the population of CD45 cells and the subtypes of T cells and natural killer cells; in contrast, the proportion of macrophages was significantly increased. There were no significant differences between the other cell subsets in each group.
The changes in immune cell subsets may be closely associated with the progression of EH. Although the specific role of different immune cell subsets in the development of the diseases requires further study, the changes in the proportions of immune cell subsets should not be ignored.
子宫内膜增生症（EH）的特征是子宫内膜腺体与基质的比例>1，是世界上最常见的妇科疾病之一。免疫细胞亚群在 EH 发展中的作用尚不清楚。
本研究纳入因异常子宫出血而行刮宫术的患者。通过流式细胞术分析患者子宫内膜中不同类型免疫细胞亚群数量的变化。
本研究共纳入 48 例患者，根据病理结果分为三组：（a）增殖期（PP，n=12）；（b）单纯性 EH（SEH，n=30）；和（c）复杂性 EH（CEH，n=6）。结果表明，这三组之间免疫细胞亚群存在显著差异。与 PP 组相比，SEH 患者的 CD45 细胞和中性粒细胞比例以及 T 细胞和巨噬细胞亚群明显增加。与 PP 和 SEH 组相比，CEH 组的免疫细胞亚群明显减少，包括 CD45 细胞群体和 T 细胞和自然杀伤细胞亚群；相反，巨噬细胞的比例明显增加。各组间其他细胞亚群无显著差异。
免疫细胞亚群的变化可能与 EH 的进展密切相关。尽管不同免疫细胞亚群在疾病发展中的具体作用需要进一步研究，但免疫细胞亚群比例的变化不容忽视。
