Barber E F, Cousins R J
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
J Nutr. 1988 Mar;118(3):375-81. doi: 10.1093/jn/118.3.375.
Regulation of ceruloplasmin synthesis by interleukin-1 (IL-1) as influenced by dietary copper status was examined in rats. In copper-sufficient rats, ceruloplasmin oxidase activity did not peak in the serum until at least 24 h after IL-1 was given. The rates of ceruloplasmin synthesis, as measured by pulse labeling with [3H]leucine and immunoprecipitation, peaked 12 h after IL-1 and returned to basal rates by 24 h. Copper had to be given to copper-deficient rats for the IL-1 to induce oxidase activity. This activity was stimulated by copper alone in the deficient rats but not to the same level as with IL-1 plus copper. Copper alone did not increase the synthesis rate. IL-1 alone stimulated the synthesis rate without increasing the oxidase activity in the copper-deficient rats. The induction pattern of ceruloplasmin synthesis by IL-1 was the same in the copper-deficient rats given copper as in copper-sufficient rats. Actinomycin D blocked the stimulation of ceruloplasmin synthesis by IL-1. It was concluded that ceruloplasmin is dependent on copper incorporation for oxidase activity, but its synthesis is induced by IL-1 regardless of dietary copper levels, probably by a mechanism involving transcriptional regulation.
研究了饮食铜状态对大鼠体内白细胞介素-1（IL-1）调节铜蓝蛋白合成的影响。在铜充足的大鼠中，直到给予IL-1后至少24小时，血清中的铜蓝蛋白氧化酶活性才达到峰值。通过用[3H]亮氨酸脉冲标记和免疫沉淀法测定，铜蓝蛋白合成速率在给予IL-1后12小时达到峰值，并在24小时恢复到基础速率。必须给缺铜大鼠补充铜，IL-1才能诱导氧化酶活性。在缺铜大鼠中，单独铜就能刺激这种活性，但不如IL-1加铜时刺激的水平高。单独铜不会增加合成速率。在缺铜大鼠中，单独IL-1能刺激合成速率，但不会增加氧化酶活性。给予铜的缺铜大鼠中，IL-1诱导铜蓝蛋白合成的模式与铜充足大鼠相同。放线菌素D阻断了IL-1对铜蓝蛋白合成的刺激。得出的结论是，铜蓝蛋白的氧化酶活性依赖于铜的掺入，但其合成由IL-1诱导，与饮食铜水平无关，可能是通过一种涉及转录调节的机制。
