Department of Health, School of Health, Zabol University of Medical Sciences, Zabol, Iran.
Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Mol Biol (Noisy-le-grand). 2020 Jun 25;66(4):270-279.
Alstonia sholaris is an evergreen tree commonly found in South East Asia. In traditional medicine pharmacological activities are attributed to the leaves and bark of this plant. The aim of this study is characterizing the chemicals present in A. sholaris leaves and bark extracts and study their antimicrobial activities. Solvent extractions with Soxhlet apparatus of leaves and bark were obtained using hexane, benzene, isopropanol, methanol, and water. The crude extracts were concentrated and screened for qualitative phytochemical analysis and thin layer chromatography, and the antibacterial, antifungal an antiviral activity of crude extracts were measured by in vitro methods. Isopropanol and methanol extracts showed significant antibacterial activity and it was more pronounced against Gram positive than against Gram negative bacteria. Hexane, benzene, isopropanol and methanol fractions of A. scholaris bark and leaf showed activity against Enterobacter cloacae. Isopropanol extract showed maximum activity against selected human pathogenic fungus. In conclusion, the leaves and bark of A. scholaris are rich in phytochemicals with antimicrobial activities against human pathogens, being the isopropanol fraction the one with the highest antibacterial, antifungal, antiviral and anti-mycobacterial activities.
轮环藤是一种常见于东南亚的常绿乔木。在传统医学中，该植物的叶子和树皮具有药理学活性。本研究的目的是对轮环藤叶和树皮提取物中的化学成分进行表征，并研究其抗菌活性。使用索氏提取器用正己烷、苯、异丙醇、甲醇和水对叶片和树皮进行溶剂萃取。粗提物浓缩后进行定性植物化学分析和薄层色谱分析，并通过体外方法测量粗提物的抗菌、抗真菌和抗病毒活性。异丙醇和甲醇提取物表现出显著的抗菌活性，对革兰氏阳性菌的活性比革兰氏阴性菌更强。轮环藤树皮和叶的正己烷、苯、异丙醇和甲醇馏分对阴沟肠杆菌均有活性。异丙醇提取物对所选的人类致病真菌表现出最大的活性。总之，轮环藤的叶子和树皮富含具有抗菌活性的植物化学物质，对人类病原体具有抗菌、抗真菌、抗病毒和抗分枝杆菌活性，其中异丙醇馏分的抗菌、抗真菌、抗病毒和抗分枝杆菌活性最高。
