Laboratory of Agrozoology, Department of Plants and Crops, Ghent University, Ghent, Belgium.
U.S. Department of Agriculture, College Station, Insect Control and Cotton Disease Research Unit, Southern Plains Agricultural Research Center, TX, USA.
Pest Manag Sci. 2020 Oct;76(10):3451-3458. doi: 10.1002/ps.5971. Epub 2020 Jul 27.
Insect Capability neuropeptides (CAP2b/CAPA-PKs) play a critical role in modulating different physiologies and behavior in insects. In a previous proof-of-concept study, the CAP2b analogues 1895 (2Abf-Suc-FGPRLamide) and 2129 (2Abf-Suc-ATPRIamide) were reported to reduce aphid fitness when administered by injection. In the current study, the insecticidal efficacy of 1895 and 2129 on the peach potato aphid Myzus persicae was analyzed by topical application, simulating a spray application scenario in the field. Additionally, the selectivity of the tested analogues was evaluated against a selection of beneficial insects, namely three natural enemies (Adalia bipunctata, Chrysoperla carnea and Nasonia vitripennis) and a pollinator (Bombus terrestris).
Within 3-5 days post topical exposure of aphids to 1895, higher mortality (33%) was observed, as was the case for the treatment with 2129 (17%) and the mixture of 1895 + 2129 (47%) compared to the control (3%). 1895 and the mix 1895 + 2129 showed the strongest and comparable insecticidal effects. Additionally, surviving aphids treated with 1895 showed a reduction in total lifetime reproduction (GRR) of 30%, 19% with 2129 and 39% with the mix 1895 + 2129. Of interest from a biosafety perspective is that by using the same delivery method and dose, no significant effects on survival, weight increase and food intake was observed for the representative natural enemies and the pollinator.
This study highlights the potential of exploiting CAP2b analogues such as 1895 (core structure FGPRL) as aphicides. Additionally, the CAP2b analogues used in this study were selective as they showed no effects when applied on four representative beneficial insects.
昆虫能力神经肽（CAP2b/CAPA-PKs）在调节昆虫的不同生理和行为方面起着关键作用。在之前的概念验证研究中，报告了 CAP2b 类似物 1895（2Abf-Suc-FGPRLamide）和 2129（2Abf-Suc-ATPRIamide）在注射给药时降低蚜虫适应性。在本研究中，通过表面施药分析了 1895 和 2129 对桃蚜 Myzus persicae 的杀虫效果，模拟了田间喷雾应用场景。此外，评估了测试类似物对一系列有益昆虫的选择性，即三种天敌（Adalia bipunctata、Chrysoperla carnea 和 Nasonia vitripennis）和一种传粉媒介（Bombus terrestris）。
在蚜虫经表面接触 1895 后 3-5 天内，与对照组（3%）相比，死亡率更高（33%），2129 处理（17%）和 1895+2129 混合物（47%）也是如此。1895 和混合 1895+2129 表现出最强和相当的杀虫效果。此外，用 1895 处理的存活蚜虫的总终生繁殖力（GRR）降低了 30%，用 2129 降低了 19%，用混合 1895+2129 降低了 39%。从生物安全的角度来看，有趣的是，使用相同的给药方法和剂量，对代表性天敌和传粉媒介的生存、体重增加和食物摄入没有观察到显著影响。
本研究强调了利用 CAP2b 类似物（如 1895（核心结构 FGPRL））作为杀蚜虫剂的潜力。此外，本研究中使用的 CAP2b 类似物具有选择性，因为它们在施用于四种代表性有益昆虫时没有效果。
