慢性鼠脑成像：从光学系统到功能超声。
Chronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound.
机构信息
Boston University Biomedical Engineering, Neurophotonics Center, Boston, Massachusetts.
出版信息
Curr Protoc Neurosci. 2020 Sep;93(1):e98. doi: 10.1002/cpns.98.
Abstract
Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke, and neurodegenerative diseases. This method can also be used with awake mice; hence, the deleterious effects of anesthesia on neurovascular responses can be avoided. In addition to fUS imaging, this surgical preparation allows researchers to take advantage of common optical imaging methods to acquire complementary datasets to help increase the technical rigor of studies. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Surgical preparation of mouse chronic cranial windows using polymethylpentene Basic Protocol 2: Imaging of mice with chronic cranial windows.
摘要
功能性超声（fUS）在脑血管成像中的应用在神经科学家中日渐流行。本文描述了一种慢性手术准备方法，该方法允许进行纵向研究，因此适用于广泛的研究，特别是关于衰老、中风和神经退行性疾病的研究。该方法也可用于清醒的小鼠；因此，可以避免麻醉对神经血管反应的有害影响。除了 fUS 成像外，这种手术准备还允许研究人员利用常见的光学成像方法获取补充数据集，以帮助提高研究的技术严谨性。© 2020 威立出版公司。 基本方案 1：使用聚甲基戊烯对小鼠慢性颅窗进行手术准备 基本方案 2：对具有慢性颅窗的小鼠进行成像。
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