State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; School of Marine Sciences, Ningbo University, Ningbo, 315211, China.
School of Civil and Environmental Engineering, Ningbo University, Ningbo, 315211, China.
Environ Pollut. 2020 Oct;265(Pt B):115047. doi: 10.1016/j.envpol.2020.115047. Epub 2020 Jun 18.
Terminating harmful algal blooms by using algicidal agents is a strong disturbance event in marine environment, which has powerful structural influences on microbial ecosystems. But, the response of microbial ecosystem to algicidal agent is largely unknown. Here, we conducted Phaeocystis globosa microcosms to investigate the dynamics, assembly processes, and co-occurrence patterns of microbial communities in response to algicidal process induced by a highly efficient algicidal agent, prodigiosin, by using 16S rRNA gene amplicon sequencing. The α-diversity of microbial community showed no obvious changes during the algicidal process in P. globosa microcosm treated with prodigiosin (group PD). Rhodobacteraceae increased significantly (P < 0.05) during algicidal process in PD, and this was mainly due to the lysis of P. globosa cells. Compared to the control group, the temporal turnover rates of common and rare taxa in PD were significantly higher because of the lysis of P. globosa induced by prodigiosin. Neutral processes mainly drove the assembly of microbial communities in all microcosms, even though the algicidal process induced by prodigiosin had no effect on the assembly processes. In addition, the time-decay relationship and co-occurrence network analysis indicate that rare taxa play important roles in maintaining microbial community stability in response to the algicidal process, rather than prodigiosin. These findings suggest that prodigiosin cannot affect the dynamics of microbial communities directly; however, future investigations into the function of microbial communities in response to prodigiosin remain imperative.
使用杀藻剂来终止有害藻类的爆发是海洋环境中的一个强烈干扰事件，它对微生物生态系统具有强大的结构影响。但是，微生物生态系统对杀藻剂的反应在很大程度上是未知的。在这里，我们通过使用 16S rRNA 基因扩增子测序，进行了聚球藻微宇宙实验，以研究在高效杀藻剂——灵菌红素作用下，微生物群落对杀藻过程的动态、组装过程和共生模式的响应。在聚球藻微宇宙中用灵菌红素处理（PD 组）期间，微生物群落的 α 多样性没有明显变化。在 PD 中的杀藻过程中，红杆菌科的数量显著增加（P<0.05），这主要是由于聚球藻细胞的裂解。与对照组相比，由于灵菌红素诱导的聚球藻裂解，PD 中常见和稀有分类单元的时间周转率明显更高。中性过程主要驱动了所有微宇宙中微生物群落的组装，即使灵菌红素诱导的杀藻过程对组装过程没有影响。此外，时间衰减关系和共生网络分析表明，稀有分类单元在响应杀藻过程维持微生物群落稳定性方面发挥着重要作用，而不是灵菌红素。这些发现表明灵菌红素不能直接影响微生物群落的动态；然而，未来对微生物群落对灵菌红素的功能的研究仍然是必要的。
