Department of Peripheral Vascular Diseases, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, China.
Department of Peripheral Vascular Diseases, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, China.
Cytokine. 2020 Sep;133:155173. doi: 10.1016/j.cyto.2020.155173. Epub 2020 Jun 22.
Diabetic foot ulcers (DFU) remain a serious public health problem. However, the current evaluation and confirmation of the efficacy of DFU is unclear. The present study aimed to measure the alterations of circulating proteins in patients after DFU healing for the exploration of the prognostic biomarkers. The serum cytokine profiles of DFU patients before and after treatment were analyzed by a human antibody microarray technology using diabetic patients and healthy population as control groups. The results showed that ten differential cytokines were associated with DFU healing. Among these ten cytokines, comparing to DFU group, nine ones (Jagged-1, CD14, Cathepsin S, Syndecan 4, MDC, TARC, Angiopoietin-4, Clusterin and HGFR) were significantly up-regulated in DFU-treated group and Follistatin-like 1 was significantly down-regulated, while their levels in DFU-treated group showed no significant differences from those in control groups. Furthermore, ELISA validation also showed that compares to DFU group four of ten (MDC, TARC, Clusterin, and Syndecan 4) were increased in DFU-treated group equal to the levels in control groups, consistent with the array results. Our finding shows that these four cytokines may have great potential for prognostic evaluation of DFU healing.
糖尿病足溃疡（DFU）仍然是一个严重的公共卫生问题。然而，目前对于 DFU 疗效的评估和确认尚不清楚。本研究旨在通过人类抗体微阵列技术测量 DFU 愈合后患者循环蛋白的变化，以探索预后生物标志物。使用糖尿病患者和健康人群作为对照组，分析了 DFU 患者治疗前后的血清细胞因子谱。结果表明，有十种差异细胞因子与 DFU 愈合有关。在这十种细胞因子中，与 DFU 组相比，Jagged-1、CD14、组织蛋白酶 S、Syndecan 4、MDC、TARC、血管生成素 4、Clusterin 和 HGFR 等九种细胞因子在 DFU 治疗组中显著上调，Follistatin-like 1 显著下调，而其在 DFU 治疗组中的水平与对照组无显著差异。此外，ELISA 验证也表明，与 DFU 组相比，在 DFU 治疗组中，有四种细胞因子（MDC、TARC、Clusterin 和 Syndecan 4）的水平与对照组相当，与阵列结果一致。我们的发现表明，这四种细胞因子可能对 DFU 愈合的预后评估具有很大的潜力。
