Department of Chemistry, Sungkyunkwan University, Suwon 16419, South Korea.
Anal Chem. 2020 Jul 21;92(14):10099-10107. doi: 10.1021/acs.analchem.0c02065. Epub 2020 Jul 9.
We report a biosensing platform based on a binary mixture comprised of Au nanorods (plasmonic nanoparticles, Au NRs) and magnetically responsive Pt@Ni nanorings (magnetic nanostirrers, MN-rings). The mixture of Au NRs and MN-rings was modulated with an external rotating magnetic field (a dynamic assay with magnetic perturbation), which led to fluctuating extinction in the UV-vis spectroscopy measurement. As the surfaces of Au NRs were modified with antigens and antibodies, their periodic profile of extinction changed in accordance with surface modification of the Au NRs. The obtained periodic extinction with time could be converted to a frequency domain function where the signal-to-noise ratios of the peaks were evaluated to monitor surface biorecognitions on Au NRs, which is in contrast to conventional biosensors (a stagnant assay without perturbation) that use only the peak shift of localized surface plasmon resonance of Au nanoparticles.
我们报告了一种基于金纳米棒（等离子体纳米粒子，AuNRs）和磁响应的 Pt@Ni 纳米环（磁性纳米搅拌器，MN-rings）的二元混合物的生物传感平台。AuNRs 和 MN-rings 的混合物通过外加旋转磁场进行调制（具有磁扰动的动态测定），这导致在紫外可见光谱测量中出现波动的消光。由于 AuNRs 的表面被抗原和抗体修饰，因此它们的消光周期性会根据 AuNRs 的表面修饰而变化。随着时间的推移获得的周期性消光可以转换为频域函数，其中可以评估峰的信噪比以监测 AuNRs 上的表面生物识别，这与传统的生物传感器（没有扰动的静态测定）形成对比，传统的生物传感器仅使用 Au 纳米粒子的局域表面等离子体共振的峰位移。
