School of Chinese Medicine, Bozhou University, Bozhou, 236800, China.
School of Chinese Medicine, Bozhou University, Bozhou, 236800, China; and Corresponding authors. Email:
Funct Plant Biol. 2020 Sep;47(10):937-944. doi: 10.1071/FP19345.
The herbal orchid Bletilla striata (Thunb.) Rchb.f. has a long cultivation history and has been widely used in medicines and cosmetics. The fungal infection leaf blight (LB) seriously threatens B. striata cultivation. Here, we systemically collected wild B. striata accessions and isolated the accessions with strong resistance against LB. We carried out proteomic profiling analysis of LB-resistant and LB-susceptible accessions, and identified a large number of differentially expressed proteins with significant gene ontology enrichment for 'oxidoreductase activity.' Of the proteins identified in the reactive oxygen species signalling pathway, the protein abundance of the Cu-Zn superoxide dismutase BsSOD1 and its gene expression level were higher in LB-resistant accessions than in LB-susceptible lines. Transient expression of the dismutase fused with yellow fluorescent protein determined that its subcellular localisation is in the cytoplasm. Our study provides new insights into the molecular markers associated with fungal infection in B. striata.
中药材白芨（Bletilla striata）（Thunb.）Rchb.f. 栽培历史悠久，在药品和化妆品中应用广泛。真菌感染引起的叶枯病（LB）严重威胁着白芨的栽培。本研究系统地收集了野生白芨资源，并分离出对 LB 具有较强抗性的白芨品种。对白芨抗 LB 和感 LB 品种进行蛋白质组学分析，鉴定到大量具有显著“氧化还原酶活性”基因本体论富集的差异表达蛋白。在活性氧信号通路中鉴定到的蛋白中，Cu-Zn 超氧化物歧化酶 BsSOD1 的蛋白丰度在抗 LB 品种中高于感 LB 品种，且其基因表达水平也更高。与黄色荧光蛋白融合的该酶的瞬时表达表明其亚细胞定位在细胞质中。本研究为与白芨真菌感染相关的分子标记提供了新的见解。
