Zhejiang Chinese Medical University, Hangzhou 310053, China.
Biomed Res Int. 2018 May 13;2018:3246398. doi: 10.1155/2018/3246398. eCollection 2018.
(Thunb.), an ornamental and medicinal plant, is on the list of endangered plants in China. Its pseudobulb is abundant in polysaccharide and has been used for centuries as a herbal remedy. However, a recent rise in demand has placed it at risk of extinction, and therefore, research on its propagation and genetic improvement is essential. Since polyploids tend to possess advantageous qualities, we incubated seeds with colchicine with the aim of creating tetraploid plantlets. Aseptic seeds treated with 0.1% colchicine for 7 days showed the highest tetraploid induction rate of 40.67 ± 0.89%. Compared with the wild-type, the tetraploids could be identified by their morphological characteristics including larger stomata at a lower density, larger leaf blades, and a thicker petiole. Contents of polysaccharide and phenolic compounds were also determined in the tetraploid pseudobulbs, revealing significantly higher values than in the wild-type. colchicine treatment can therefore be used to successfully produce tetraploids with superior pseudobulbs.
(Thunb.)，一种观赏和药用植物，被列入中国濒危植物名单。其假鳞茎富含多糖，几个世纪以来一直被用作草药。然而，最近需求的增加使其面临灭绝的危险，因此，对其繁殖和遗传改良的研究至关重要。由于多倍体往往具有优势品质，我们用秋水仙素孵育种子，旨在培育四倍体苗。用 0.1%秋水仙素处理 7 天的无菌种子显示出最高的四倍体诱导率为 40.67±0.89%。与野生型相比，四倍体可以通过其形态特征来识别，包括气孔密度较低、叶片较大和叶柄较厚。四倍体假鳞茎中的多糖和酚类化合物含量也有所测定，发现明显高于野生型。因此，秋水仙素处理可成功用于生产具有优良假鳞茎的四倍体。
