补充葡萄糖醛酸和 N-乙酰-D-葡萄糖胺对猪卵母细胞 IVM 期间卵周隙的影响。

Effects of glucuronic acid and N-acetyl-D-glucosamine supplementation on the perivitelline space during the IVM of pig oocytes.

机构信息

Department of Animal and Pre-veterinary Studies, University of Findlay, 1000 N. Main Street, Findlay, OH 45840, USA.

Department of Animal and Pre-veterinary Studies, University of Findlay, 1000 N. Main Street, Findlay, OH 45840, USA; and Corresponding author. Email:

出版信息

Reprod Fertil Dev. 2020 Jun;32(10):941-947. doi: 10.1071/RD20109.

DOI:10.1071/RD20109

PMID:32586424

Abstract

The objective of this study was to minimise polyspermic penetration by increasing the perivitelline space (PVS) thickness through supplementation of the hyaluronic acid components glucuronic acid and N-acetyl-d-glucosamine (GlcNAc). Oocytes (n=4690) were supplemented during the first 24h and/or the remainder of maturation (final 16-18h) with 0.01mM glucuronic acid and 0.01mM GlcNAc and then evaluated for PVS thickness, hyaluronic acid, glutathione and glutathione peroxidase concentrations. Fertilised oocytes were evaluated for polyspermic penetration and embryo development. The PVS thickness and amount of hyaluronic acid was significantly (P<0.05) greater in oocytes supplemented with 0.01mM glucuronic acid and 0.01mM GlcNAc during the second part or all of maturation compared with the other treatments. In addition, polyspermic penetration was significantly (P<0.05) less in oocytes supplemented with 0.01mM glucuronic acid and 0.01mM GlcNAc during the second part or all of maturation compared with the other treatments. Supplementing 0.01mM glucuronic acid and GlcNAc during maturation significantly (P<0.05) increased the percentage of cleaved embryos by 48h after IVF and blastocysts formed by 144h after IVF compared those not supplemented. These results indicate that supplementing PVS components during maturation decreases polyspermic penetration by increasing PVS thickness.

摘要

本研究旨在通过增加透明带间隙（PVS）厚度来减少多精受精，具体方法是添加透明质酸成分葡萄糖醛酸和 N-乙酰-D-葡萄糖胺（GlcNAc）。在培养的前 24 小时和/或其余的成熟阶段（最后 16-18 小时），向卵母细胞中添加 0.01mM 葡萄糖醛酸和 0.01mM GlcNAc，然后评估 PVS 厚度、透明质酸、谷胱甘肽和谷胱甘肽过氧化物酶浓度。对受精后的卵母细胞进行多精受精穿透和胚胎发育评估。与其他处理组相比，在成熟的第二部分或全部添加 0.01mM 葡萄糖醛酸和 0.01mM GlcNAc 的卵母细胞中，PVS 厚度和透明质酸含量显著增加（P<0.05）。此外，与其他处理组相比，在成熟的第二部分或全部添加 0.01mM 葡萄糖醛酸和 0.01mM GlcNAc 的卵母细胞中，多精受精穿透率显著降低（P<0.05）。在成熟过程中添加 0.01mM 葡萄糖醛酸和 GlcNAc 可使 IVF 后 48 小时的分裂胚胎百分比显著增加（P<0.05），并使 IVF 后 144 小时形成的囊胚百分比增加，而未添加的则无此效果。这些结果表明，在成熟过程中添加 PVS 成分可通过增加 PVS 厚度来减少多精受精穿透。