Institute of Molecular and Cell Biology, A*STAR Research Entities, Singapore; Department of Biological Sciences, National University of Singapore, Singapore.
Institute of Molecular and Cell Biology, A*STAR Research Entities, Singapore; Yong Loo Lin School of Medicine (Physiology), National University of Singapore, Singapore; The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; National Neuroscience Institute, Singapore.
Methods Cell Biol. 2020;159:257-277. doi: 10.1016/bs.mcb.2020.03.010. Epub 2020 Apr 21.
Current advances in human pluripotent stem cell (hPSC) technology allow directed differentiation into three-dimensional spinal organoid cultures that mimic the unique microenvironment and cytoarchitecture of the human spinal cord. Organoids also serves as important cellular tools to model spinal cord development and motor neuron diseases such as Spinal Muscular Atrophy and Amyotrophic Lateral Sclerosis. In this chapter, we describe a detailed step-by-step methodology to generate spinal organoids from human PSCs. We include advantages and limitations of the use of spinal organoids and notes on technical and design considerations in generating these spinal organoids.
目前，人类多能干细胞（hPSC）技术的进展使得定向分化为三维脊髓类器官培养成为可能，这种培养物可以模拟人类脊髓的独特微环境和细胞结构。类器官也是模拟脊髓发育和运动神经元疾病（如脊髓性肌萎缩症和肌萎缩性侧索硬化症）的重要细胞工具。在本章中，我们描述了从人类 PSC 生成脊髓类器官的详细分步方法。我们包括了使用脊髓类器官的优缺点，并介绍了在生成这些脊髓类器官时的技术和设计注意事项。
