Romanov V I, Sorokin A B, Nesterov A M, Komissarchik Ia Iu
Tsitologiia. 1988 Jan;30(1):34-8.
The plasma membrane ultrastructural changes after the action of epidermal growth factor were studied in A-431 cells using freeze-fracture methods. The incubation with EGF (100 ng/ml, 0 degree C, 60 min) led to a decrease in density of intramembrane particles on the P surface of ventral cell membrane, while the number of coated pits increased there. The revealed effects of EGF may be related to direct consequences of EGF-receptor complex formation, because all the temperature dependent steps of its processing were blocked. The data obtained testify to an active involvement of the membrane ventral surface in the formation of cell response towards growth factors.
采用冷冻蚀刻法研究了表皮生长因子作用于A - 431细胞后质膜的超微结构变化。用表皮生长因子（100 ng/ml，0℃，60分钟）孵育导致腹侧细胞膜P面的膜内颗粒密度降低，而此处的被膜小窝数量增加。所揭示的表皮生长因子的作用可能与表皮生长因子 - 受体复合物形成的直接后果有关，因为其加工过程中所有依赖温度的步骤均被阻断。所得数据证明膜腹面积极参与细胞对生长因子反应的形成。
