Postgraduate Program in Dentistry, School of Dentistry, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil.
Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil.
Sci Rep. 2020 Jun 25;10(1):10311. doi: 10.1038/s41598-020-67303-z.
This study aimed to compare the effect of conventional irrigation (CI) and passive ultrasonic irrigation (PUI) with 17% EDTA and QMiX on the maximum depth and percentage of sealer penetration into the dentinal tubules by confocal laser scanning microscopy (CLSM) and to describe the cleaning of root canal walls by scanning electron microscopy (SEM). Eighty single-rooted human mandibular premolars were instrumented and randomly assigned to four groups (n = 20): EDTA + CI, QMiX + CI, EDTA + PUI, and QMiX + PUI. Ten samples from each group were examined by SEM (2,000×) and the remaining 40 roots were filled with a single gutta-percha cone and AH Plus sealer mixed with 0.1% rhodamine B for analysis by CLSM (10×). Images were assessed at distances of 2 mm (apical), 5 mm (middle), and 8 mm (coronal) from the apex with the Leica Application Suite V4.10 software. The EDTA + PUI and QMiX + PUI protocols presented higher rates of debris/smear layer removal in the apical and middle thirds. The PUI was superior to CI in the maximum depth of sealer penetration at the middle third. The QMiX + PUI group had a higher percentage of sealer penetration at the apical third. The PUI and QMiX protocol improved debris/smear layer removal and tubular dentin sealer penetration.
本研究旨在通过共聚焦激光扫描显微镜 (CLSM) 比较传统冲洗 (CI) 和被动超声冲洗 (PUI) 联合 17% EDTA 和 QMiX 与 PUI 联合 EDTA 和 QMiX 对牙本质小管内封闭剂渗透最大深度和百分比的影响，并通过扫描电子显微镜 (SEM) 描述根管壁的清洁情况。80 颗单根下颌前磨牙被仪器化并随机分为四组（n = 20）：EDTA + CI、QMiX+CI、EDTA + PUI 和 QMiX+PUI。每组的 10 个样本用 SEM（2,000×）进行检查，其余 40 个根用单个牙胶尖和 AH Plus 密封剂填充，并与 0.1%罗丹明 B 混合，用于 CLSM（10×）分析。图像在距离根尖 2 mm（根尖）、5 mm（中间）和 8 mm（冠部）处用 Leica Application Suite V4.10 软件进行评估。EDTA + PUI 和 QMiX+PUI 方案在根尖和中间三分之一处具有更高的去除碎屑/玷污层的比例。在中间三分之一处，PUI 比 CI 具有更高的密封剂渗透最大深度。QMiX+PUI 组在根尖三分之一处具有更高的密封剂渗透比例。PUI 和 QMiX 方案提高了碎屑/玷污层的去除和管状牙本质密封剂的渗透。
