Department of Endodontics, Faculty of Dentistry, Abant İzzet Baysal University, Bolu, Turkey.
Department of Endodontics, Faculty of Dentistry, Ondokuz Mayıs University, Samsun, Turkey.
Odontology. 2019 Jan;107(1):64-71. doi: 10.1007/s10266-018-0359-0. Epub 2018 Apr 12.
The aim of the present study was to compare the effect of chitosan nanoparticle, QMix, and 17% EDTA on the penetrability of a calcium silicate-based sealer into dentinal tubules using a confocal laser scanning microscope (CLSM). Sixty mandibular premolar teeth were selected and randomly divided into three groups (n = 20) before root canal preparation according to the solution used in the final rinse protocol: chitosan, QMix, and EDTA groups. Twenty teeth of each group were filled with a TotalFill BC sealers' single gutta-percha cone and with 0.1% rhodamine B. The specimens were horizontally sectioned at 3 and 5 mm from the apex, and the slices were analyzed in CLSM (4×). Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy with using Image J analysis software. Dentinal tubule's penetration depth, percentage, and area were measured using imaging software. Kruskal-Wallis test was used for statistical analysis. The level of significance was set at 5%. Results of Kruskal-Wallis analysis showed that there was a significant difference in the percentage and depth of sealer penetration among all groups at 3 and 5 mm level sections (P < 0.05). Within the groups, the minimum sealer penetration depth was recorded for chitosan nanoparticle group. Greater depth of sealer penetration was recorded at 5 mm as compared to 3 mm in all the groups. Within the limitation of the present study, it can be concluded that QMix and EDTA promoted sealer penetration superior to that achieved by chitosan nanoparticle.
本研究旨在比较壳聚糖纳米粒子、QMix 和 17%EDTA 对使用共聚焦激光扫描显微镜（CLSM）检测的硅酸钙基封闭剂进入牙本质小管的渗透性的影响。在根管预备前，根据最终冲洗方案中使用的溶液，将 60 颗下颌前磨牙随机分为三组（n=20）：壳聚糖组、QMix 组和 EDTA 组。每组的 20 颗牙齿用 TotalFill BC 密封剂的单个牙胶尖和 0.1%罗丹明 B 填充。将样本从根尖水平 3 和 5mm 处水平切片，使用共聚焦激光扫描显微镜（4×）分析切片。使用 Image J 分析软件测量用共聚焦激光扫描显微镜测量的总渗透百分率和最大密封剂渗透深度。使用成像软件测量牙本质小管的渗透深度、百分率和面积。采用 Kruskal-Wallis 检验进行统计学分析。显著性水平设定为 5%。Kruskal-Wallis 分析结果显示，在 3 和 5mm 水平切片的所有组中，密封剂渗透百分率和深度均存在显著差异（P<0.05）。在组内，壳聚糖纳米粒子组的密封剂渗透深度最小。与 3mm 相比，所有组在 5mm 处的密封剂渗透深度更深。在本研究的限制范围内，可以得出结论，QMix 和 EDTA 促进了密封剂的渗透，优于壳聚糖纳米粒子。
