Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Behav Neurol. 2020 Jun 7;2020:4813616. doi: 10.1155/2020/4813616. eCollection 2020.
Mouse neonates were exposed to MS paradigm 3 hours daily from postnatal days (PND) 2 to 14. The control and MS mice were divided separately into 16 groups ( = 8) (8 groups for each set) including mice that received normal saline, mice that received rutin at doses of 10, 50, and 100 mg/kg, mice that received NMDA at a dose of 150 mg/kg, mice that received ketamine (NMDA antagonist) at a dose of 0.25 mg/kg, mice that received NMDA antagonist plus a subeffective dose of rutin, and mice that received NMDA plus an effective dose of rutin. Forced swimming test (FST) was performed. Afterwards, the hippocampus was evaluated in cases of histopathological changes as well as expression of NR2A and NR2B genes.
Rutin significantly reduced immobility time in the FST. The expression of NR2A and NR2B subunits of NMDA receptor in MS mice was significantly higher than that in the control group. Rutin significantly decreased the expression of NR2B and NR2A subunits in the hippocampus. The CA3 diameter and percentage of dark neurons in the hippocampus of MS mice significantly decreased and increased, respectively, which partially reversed following rutin administration.
Rutin, partially, through a neuroprotective effect on the hippocampus exerted antidepressant-like effect. We concluded that NMDA receptors, at least in part, mediated the beneficial effect of rutin.
将新生小鼠从出生后第 2 天到第 14 天，每天暴露于 MS 范式 3 小时。对照组和 MS 组小鼠分别分为 16 组（每组 8 只）（每组 8 只），包括接受生理盐水、10、50 和 100mg/kg 芦丁、150mg/kg NMDA、0.25mg/kg 氯胺酮（NMDA 拮抗剂）、NMDA 拮抗剂加芦丁亚效剂量和 NMDA 加芦丁有效剂量的小鼠。进行强迫游泳试验（FST）。之后，评估海马组织的病理变化以及 NMDA 受体 NR2A 和 NR2B 基因的表达。
芦丁显著减少 FST 中的不动时间。MS 小鼠 NMDA 受体 NR2A 和 NR2B 亚基的表达明显高于对照组。芦丁显著降低海马中 NR2B 和 NR2A 亚基的表达。MS 小鼠海马 CA3 直径和暗神经元百分比分别显著降低和增加，芦丁给药后部分逆转。
芦丁部分通过对海马的神经保护作用发挥抗抑郁样作用。我们得出结论，NMDA 受体至少部分介导了芦丁的有益作用。
