原代人乳腺肿瘤细胞培养物对单克隆抗体-重组蓖麻毒素A链免疫毒素的反应
Response of primary human mammary tumor cell cultures to a monoclonal antibody-recombinant ricin A chain immunotoxin.
作者信息
Bjorn M J, Smith H S, Dairkee S H
机构信息
Cetus Corporation, Department of Protein Chemistry, Emeryville, CA 94608.
出版信息
Cancer Immunol Immunother. 1988;26(2):121-4. doi: 10.1007/BF00205604.
Malignant epithelial tumor cells were isolated and cultured from ten human mammary specimens of cancerous origin. The 260F9 monoclonal antibody (MAB) bound to frozen sections of all of the human breast tumors tested and to primary cultured cells from the tumors. Cultured cells from all ten breast tumors were sensitive to the clonal inhibitory effects of immunotoxin 260F9 MAB-recombinant ricin A chain. At an immunotoxin concentration of 200 ng/ml (about 1 nM), inhibition of colony formation was greater than 99% for all ten tumors.
从十个源自癌性的人类乳腺标本中分离并培养出恶性上皮肿瘤细胞。260F9单克隆抗体（MAB）与所有测试的人类乳腺肿瘤的冰冻切片以及肿瘤的原代培养细胞结合。来自所有十个乳腺肿瘤的培养细胞对免疫毒素260F9 MAB重组蓖麻毒素A链的克隆抑制作用敏感。在免疫毒素浓度为200 ng/ml（约1 nM）时，所有十个肿瘤的集落形成抑制率均大于99%。
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