Stampfer M, Hallowes R C, Hackett A J
In Vitro. 1980 May;16(5):415-25. doi: 10.1007/BF02618365.
Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components. Epithelial outgrowth from these organoids was greatly enhanced by the addition of conditioned medium from other human epithelial and myoepithelial cell lines. Additionally, the mammary epithelial growth was stimulated by insulin, hydrocortisone, epidermal growth factor, and steroid hormones. With this enriched nutritional environment, active cell division could be maintained for 1 to 3 months and cells could be serially subcultured 1 to 4 times.
缩乳术组织被用于获取人上皮细胞群体的短期培养物。用胶原酶和透明质酸酶消化组织产生了类似于导管和腺泡结构的细胞簇（类器官）；这些细胞簇可通过过滤与基质成分分离。添加来自其他人上皮和肌上皮细胞系的条件培养基可极大地促进这些类器官的上皮细胞生长。此外，胰岛素、氢化可的松、表皮生长因子和类固醇激素可刺激乳腺上皮生长。在这种丰富的营养环境下，活跃的细胞分裂可维持1至3个月，细胞可连续传代培养1至4次。
