Metcalf D, Nicola N A
Cancer Research Unit, Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Victoria, Australia.
Proc Natl Acad Sci U S A. 1988 May;85(9):3160-4. doi: 10.1073/pnas.85.9.3160.
The hemopoietic regulator multipotential colony-stimulating factor [Multi-CSF (interleukin 3)] has proliferative effects on a wide range of hemopoietic cells in vitro and in vivo. Native or recombinant Multi-CSF injected intravenously into adult mice had an initial half-life of 3-5 min and a second phase of 50 min. Clear labeling of hemopoietic cells was observed in the bone marrow and spleen of mice injected intravenously with recombinant 125I-labeled Multi-CSF showing that injected Multi-CSF can obtain access to such cells in situ. A high proportion of injected 125I-labeled Multi-CSF of both types became localized in the liver and in the kidney (in cells of the Bowman's capsule and proximal renal tubules). The kidney appeared to be an active site of degradation of Multi-CSF with the early appearance of low molecular weight labeled material in the urine.
造血调节因子多能集落刺激因子[多集落刺激因子（白细胞介素3）]在体外和体内对多种造血细胞具有增殖作用。将天然或重组的多集落刺激因子静脉注射到成年小鼠体内，其初始半衰期为3 - 5分钟，第二阶段为50分钟。给小鼠静脉注射重组的125I标记的多集落刺激因子后，在其骨髓和脾脏中观察到造血细胞有明显的标记，这表明注射的多集落刺激因子能够在原位进入这些细胞。两种类型的注射用125I标记的多集落刺激因子中，很大一部分都定位在肝脏和肾脏（在鲍曼囊和近端肾小管的细胞中）。肾脏似乎是多集落刺激因子降解的活跃部位，尿液中早期就出现了低分子量的标记物质。
