School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310007, China.
J Ethnopharmacol. 2020 Oct 28;261:112992. doi: 10.1016/j.jep.2020.112992. Epub 2020 Jun 23.
Osteoporosis and Alzheimer's disease (AD) are both senile diseases, which are closely related to oxidative stress. Bajitianwan (BJTW) is a classic Chinese formulation consisting of seven herbal drugs: the root of Morinda officinalis F.C.How., root and rhizome of Acorus tatarinowii Schott, the root bark of Lycium chinense Mill., the sclerotium of Poria cocos (Schw.) Wolf, the root of Polygala tenuifolia Willd., sclerotium with host wood of Poria cocos (Schw.) Wolf and root and rhizome of Panax ginseng C. A. Mey. BJTW has been used for the treatment of osteoporosis and AD for hundreds of years.
This study aimed to investigate the protective effects of BJTW in the amelioration of memory impairment and bone loss induced by D-galactose and to explore the underlying mechanism.
The aging model was established in male Wistar rats by subcutaneous injection of D-galactose (100 mg/kg), and the rats were treated with huperzine-A, alendronate sodium, or the aqueous extract of BJTW for 4 months. Cognitive performance was evaluated with the Morris water maze. Rat femurs were scanned using microcomputed tomography to obtain three-dimensional imagery of bone microstructure. The impact of D-galactose on the expression of Forkhead box O1 and superoxide dismutase 2 in femur tissue was also evaluated.
For the model group, BJTW treatment significantly reduced the latency time for finding the target platform in the directional swimming test and increased time spent swimming in the target quadrant with the probe test. Additionally, BJTW treatment alleviated D-galactose-induced bone loss through regulation of levels of alkaline phosphatase, osteocalcin, osteoprotegerin, and receptor activator of nuclear factor kappa B ligand. Furthermore, BJTW treatment increased catalase and glutathione peroxidase levels in serum, reduced malondialdehyde content in hippocampus, and upregulated expression of Forkhead O1, which upregulated superoxide dismutase 2 in the femur.
BJTW had positive effects on age-related memory impairments and bone loss. It may be a promising antioxidant candidate for treatment of Alzheimer's disease and osteoporosis.
骨质疏松症和阿尔茨海默病（AD）都是老年性疾病，与氧化应激密切相关。巴戟天丸（BJTW）是一种由七种草药组成的经典中药配方：巴戟天（Morinda officinalis F.C.How.）的根、菖蒲（Acorus tatarinowii Schott）的根茎、枸杞（Lycium chinense Mill.）的根皮、茯苓（Poria cocos（Schw.）Wolf）的菌核、远志（Polygala tenuifolia Willd.）的根、茯苓（Poria cocos（Schw.）Wolf）的菌核与宿主木材、以及人参（Panax ginseng C. A. Mey.）的根和根茎。BJTW 已被用于治疗骨质疏松症和 AD 已有数百年的历史。
本研究旨在探讨 BJTW 对 D-半乳糖诱导的记忆障碍和骨丢失的改善作用，并探讨其潜在机制。
雄性 Wistar 大鼠皮下注射 D-半乳糖（100mg/kg）建立衰老模型，用石杉碱甲、阿仑膦酸钠或 BJTW 水提物治疗 4 个月。采用 Morris 水迷宫评估认知表现。采用微计算机断层扫描技术对大鼠股骨进行扫描，获得骨微结构的三维图像。还评估了 D-半乳糖对股骨组织中叉头框 O1 和超氧化物歧化酶 2 表达的影响。
对于模型组，BJTW 治疗显著缩短了定向游泳试验中寻找目标平台的潜伏期，并增加了探测试验中在目标象限游泳的时间。此外，BJTW 治疗通过调节碱性磷酸酶、骨钙素、骨保护素和核因子 kappa B 受体激活剂配体的水平缓解了 D-半乳糖引起的骨丢失。此外，BJTW 治疗增加了血清中过氧化氢酶和谷胱甘肽过氧化物酶的水平，降低了海马中丙二醛的含量，并上调了股骨中叉头 O1 的表达，从而上调了超氧化物歧化酶 2 的表达。
BJTW 对与年龄相关的记忆障碍和骨丢失有积极作用。它可能是治疗阿尔茨海默病和骨质疏松症的一种有前途的抗氧化候选药物。
