在114株耐药菌株的β-内酰胺酶特性鉴定中，寡核苷酸探针（TEM-1、OXA-1）与等电聚焦法的比较
Oligonucleotide probes (TEM-1, OXA-1) versus isoelectric focusing in beta-lactamase characterization of 114 resistant strains.
作者信息
Ouellette M, Paul G C, Philippon A M, Roy P H
机构信息
Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Ste-Foy, Quebec, Canada.
出版信息
Antimicrob Agents Chemother. 1988 Mar;32(3):397-9. doi: 10.1128/AAC.32.3.397.
Abstract
Oligonucleotide probes specific for detection of the TEM-1 and OXA-1 beta-lactamase genes were compared with isoelectric focusing in 114 gram-negative beta-lactamase-producing strains representing at least 16 species. Correlations of 96 and 100% with isoelectric points were found for the TEM-1 and OXA-1 probes, respectively.
摘要
在114株代表至少16个菌种的产β-内酰胺酶革兰氏阴性菌株中，将用于检测TEM-1和OXA-1β-内酰胺酶基因的寡核苷酸探针与等电聚焦法进行了比较。发现TEM-1和OXA-1探针与等电点的相关性分别为96%和100%。
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