• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用短生物素标记探针检测特定DNA序列。

Detection of specific DNA sequences with short biotin-labeled probes.

作者信息

Chu B C, Orgel L E

出版信息

DNA. 1985 Aug;4(4):327-31. doi: 10.1089/dna.1985.4.327.

DOI:10.1089/dna.1985.4.327
PMID:4042814
Abstract

We have developed a simple, general synthesis of nonradioactive DNA probes in which biotin is attached to the 5'-terminal phosphate of an oligodeoxyribonucleotide 16 bases long via an ethylenediamine or hexamethylenediamine linker. The products are stable under normal hybridization conditions. They hybridize to target DNA as efficiently as the underivatized oligodeoxyribonucleotide. Color development, using a commercially available kit, is complete within 3 hr using the biotin-detection method. The sensitivity of detection of homologous DNA with a probe to which biotin was attached via a hexamethylenediamine linker is about one-tenth of that achieved overnight by autoradiography with the corresponding 32P-labeled probe.

摘要

我们已经开发出一种简单、通用的非放射性DNA探针合成方法，其中生物素通过乙二胺或六亚甲基二胺连接体连接到16个碱基长的寡脱氧核糖核苷酸的5'-末端磷酸上。产物在正常杂交条件下稳定。它们与目标DNA杂交的效率与未衍生化的寡脱氧核糖核苷酸相同。使用市售试剂盒，采用生物素检测法，3小时内即可完成显色。通过六亚甲基二胺连接体连接生物素的探针检测同源DNA的灵敏度约为用相应的32P标记探针进行放射自显影过夜所达到灵敏度的十分之一。

相似文献

1
Detection of specific DNA sequences with short biotin-labeled probes.用短生物素标记探针检测特定DNA序列。
DNA. 1985 Aug;4(4):327-31. doi: 10.1089/dna.1985.4.327.
2
[Demonstration of the influenza virus A RNA by nucleic acid molecular hybridization using biotin-treated probes].[使用生物素处理的探针通过核酸分子杂交法对甲型流感病毒RNA进行的检测]
Vopr Virusol. 1990 Nov-Dec;35(6):464-6.
3
Detection of leptospiral DNA by nucleic acid hybridisation with 32P- and biotin-labelled probes.通过与32P标记和生物素标记的探针进行核酸杂交检测钩端螺旋体DNA。
J Med Microbiol. 1986 Aug;22(1):23-8. doi: 10.1099/00222615-22-1-23.
4
Comparison of radio-labeled DNA probe with a nonisotopic probe for assay of serum hepatitis B virus DNA.放射性标记DNA探针与非同位素探针检测血清乙肝病毒DNA的比较。
Clin Physiol Biochem. 1985;3(4):174-83.
5
A comparison of biotin- and 35S-based in situ hybridization methodologies for detection of human papillomavirus DNA.基于生物素和35S的原位杂交方法检测人乳头瘤病毒DNA的比较
Lab Invest. 1989 Oct;61(4):471-6.
6
Comparison of radioactive (32P and 35S) and biotinylated probes for detection of cytomegalovirus DNA.
Clin Biochem. 1987 Aug;20(4):231-5. doi: 10.1016/s0009-9120(87)80003-6.
7
Simple chemical synthesis and hybridization properties of non-radioactive DNA probes.非放射性DNA探针的简单化学合成及杂交特性
Biochimie. 1989 Mar;71(3):319-24. doi: 10.1016/0300-9084(89)90003-5.
8
Nucleic acid hybridization in the diagnosis of viral infections.核酸杂交在病毒感染诊断中的应用
Clin Lab Med. 1985 Sep;5(3):513-29.
9
Digital chemiluminescence imaging of DNA sequencing blots using a charge-coupled device camera.使用电荷耦合器件相机对DNA测序印迹进行数字化学发光成像。
Nucleic Acids Res. 1992 Dec 25;20(24):6657-65. doi: 10.1093/nar/20.24.6657.
10
Detection of Campylobacter pylori DNA by hybridisation with non-radioactive probes in comparison with a 32P-labelled probe.与32P标记探针相比，用非放射性探针杂交检测幽门螺杆菌DNA。
J Med Microbiol. 1988 Aug;26(4):257-63. doi: 10.1099/00222615-26-4-257.

引用本文的文献

1
The use of gel electrophoresis to study the reactions of activated amino acids with oligonucleotides.
J Mol Evol. 1994;38:561-5. doi: 10.1007/BF00175875.
2
1,N6-etheno deoxy and ribo adenosine and 3,N4-etheno deoxy and ribo cytidine phosphoramidites. Strongly fluorescent structures for selective introduction in defined sequence DNA and RNA molecules.1,N6-乙撑脱氧和核糖腺苷以及3,N4-乙撑脱氧和核糖胞苷亚磷酰胺。用于以特定序列选择性引入DNA和RNA分子的强荧光结构。
Nucleic Acids Res. 1994 Apr 11;22(7):1296-304. doi: 10.1093/nar/22.7.1296.
3
Reverse Southern hybridization.反向Southern杂交
Nucleic Acids Res. 1986 Dec 22;14(24):9943-54. doi: 10.1093/nar/14.24.9943.
4
A simple and efficient procedure for the synthesis of 5'-aminoalkyl oligodeoxynucleotides.一种合成5'-氨基烷基寡脱氧核苷酸的简单高效方法。
Nucleic Acids Res. 1986 Oct 24;14(20):7985-94. doi: 10.1093/nar/14.20.7985.
5
Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.将非放射性标记物连接到合成寡脱氧核糖核苷酸5'末端的高效方法。
Nucleic Acids Res. 1986 Aug 11;14(15):6227-45. doi: 10.1093/nar/14.15.6227.
6
Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.寡脱氧核苷酸-碱性磷酸酶缀合物的制备及其作为杂交探针的用途。
Nucleic Acids Res. 1986 Aug 11;14(15):6115-28. doi: 10.1093/nar/14.15.6115.
7
Synthesis of oligodeoxyribonucleotide with aliphatic amino or phosphate group at the 5' end by the phosphotriester method on a polystyrene support.在聚苯乙烯载体上通过磷酸三酯法合成5'端带有脂肪族氨基或磷酸基团的寡脱氧核糖核苷酸。
Nucleic Acids Res. 1987 Aug 11;15(15):6209-24. doi: 10.1093/nar/15.15.6209.
8
The synthesis of protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; applications of 5'-amino-oligodeoxyribonucleotides.保护型5'-氨基-2',5'-二脱氧核糖核苷-3'-O-亚磷酰胺的合成；5'-氨基寡脱氧核糖核苷酸的应用。
Nucleic Acids Res. 1987 Aug 11;15(15):6181-96. doi: 10.1093/nar/15.15.6181.
9
Covalent attachment of oligonucleotides to solid supports.寡核苷酸与固体支持物的共价连接。
Nucleic Acids Res. 1987 Jul 10;15(13):5353-72. doi: 10.1093/nar/15.13.5353.
10
Efficient methods for attachment of thiol specific probes to the 3'-ends of synthetic oligodeoxyribonucleotides.将硫醇特异性探针连接到合成寡脱氧核糖核苷酸3'末端的有效方法。
Nucleic Acids Res. 1987 Jul 10;15(13):5305-21. doi: 10.1093/nar/15.13.5305.