Fearon E R, Itaya T, Hunt B, Vogelstein B, Frost P
Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cancer Res. 1988 Jun 1;48(11):2975-80.
Transfection of the undifferentiated murine colon carcinoma line CT-26 with the gene coding for the hemagglutination antigen (HA) of influenza virus resulted in the generation of highly immunogenic tumor cells. CT-26 cells transfected with HA not only failed to grow in syngeneic mice but also protected normal animals against a challenge with otherwise lethal doses of parental nontransfected cells. The immunogenicity of HA-transfected cells appeared to correlate with surface HA expression in that tumorigenic clones of HA-transfected CT-26 cells expressed little HA, while immunogenic clones were high expressers of HA. Irradiation of immunogenic HA clones did not abrogate their immunogenicity. These observations demonstrate that immune recognition of a poorly immunogenic tumor can be produced by immunization with tumor cells expressing a defined, foreign cell surface antigen.
用编码流感病毒血凝素抗原（HA）的基因转染未分化的小鼠结肠癌细胞系CT - 26，产生了高度免疫原性的肿瘤细胞。用HA转染的CT - 26细胞不仅在同基因小鼠中无法生长，还能保护正常动物免受否则会致死剂量的亲本未转染细胞的攻击。HA转染细胞的免疫原性似乎与表面HA表达相关，因为HA转染的CT - 26细胞的致瘤性克隆表达很少的HA，而免疫原性克隆是HA的高表达者。对免疫原性HA克隆进行照射并没有消除它们的免疫原性。这些观察结果表明，通过用表达特定外来细胞表面抗原的肿瘤细胞进行免疫，可以产生对低免疫原性肿瘤的免疫识别。
