Weilemann F, Steinmetz A, Kirsch M, Buttler A, Kunze S, Kuhlisch E, Schackert H K, Schackert G
Klinik und Poliklinik für Neurochirurgie, Carl Gustav Carus Klinikum der Technischen Universität, Dresden, Germany.
Zentralbl Neurochir. 2003;64(2):65-70. doi: 10.1055/s-2003-40374.
Expression of hemagglutinin antigen of influenza virus (HA) by the murine colon carcinoma cell line (CT-26) produces systemic immunization against tumor challenges in the cecum, liver and lungs but not in the brain of BALB/c-mice. Immunization with IL-4 expressing CT-26 cells inhibits lung metastases formation. The purpose of our study was to examine the effects of HA or IL-4 expression on brain metastases formation.
Using selective internal carotid artery injections, brain metastases formation of HA or IL-4 expressing CT-26 cells with and without subcutaneous pre-immunization was evaluated in Balb/c mice.
Systemic pre-immunization with HA or IL-4 expressing tumor cells cannot protect against brain metastases, while the local, intracerebral expression of HA or IL-4 inhibits the growth of hematogenous brain metastases.
Pre-immunization with HA or IL-4 expressing tumor cells did produce systemic immunity against liver and lung metastases but not against brain metastases. Local, intracerebral expression of HA or IL-4 prevents from cerebral metastases formation in an animal model.
小鼠结肠癌细胞系（CT-26）表达流感病毒血凝素抗原（HA）可引发针对盲肠、肝脏和肺部肿瘤攻击的全身免疫，但对BALB/c小鼠的脑部无效。用表达IL-4的CT-26细胞免疫可抑制肺转移的形成。我们研究的目的是检测HA或IL-4表达对脑转移形成的影响。
通过选择性颈内动脉注射，在Balb/c小鼠中评估表达HA或IL-4的CT-26细胞在有无皮下预免疫情况下的脑转移形成情况。
用表达HA或IL-4的肿瘤细胞进行全身预免疫不能预防脑转移，而HA或IL-4在脑内的局部表达可抑制血行性脑转移的生长。
用表达HA或IL-4的肿瘤细胞进行预免疫确实产生了针对肝转移和肺转移的全身免疫，但对脑转移无效。在动物模型中，HA或IL-4在脑内的局部表达可预防脑转移的形成。
