微流控纸基分析装置用于组氨酸测定。

Microfluidic Paper-Based Analytical Device for Histidine Determination.

机构信息

Department of Biomedical Information Sciences, Graduate School of Information Sciences, Hiroshima City University, 3-4-1 Ozuka-higashi, Asaminami-ku, Hiroshima, 731-3194, Japan.

Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, FL, 32611-6250, USA.

出版信息

Appl Biochem Biotechnol. 2020 Nov;192(3):812-821. doi: 10.1007/s12010-020-03365-z. Epub 2020 Jun 27.

DOI:10.1007/s12010-020-03365-z

PMID:32592084

Abstract

A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective.

摘要

一种基于层压纸的分析器件（LPAD），用于组氨酸检测，由色谱滤纸和层压薄膜制成。组氨酸的识别是通过组氨酰-tRNA 合成酶（HisRS）实现的，其检测是基于钼蓝反应进行比色信号。考察了组氨酸的分析条件和检测浓度范围。该方法提供了 1 至 100 μM 组氨酸的选择性定量分析。LPAD 的制作非常简单，只需要对色谱滤纸和层压薄膜进行裁剪即可，并且具有成本效益。