Department of Gynaecology, Shen Zhen People's Hospital, the Second Clinical Medical College of Jinan University, Shenzhen, China.
Department of Gynaecology, the First Affiliated Hospital of Jinan University, Guangzhou, China.
J Cell Mol Med. 2020 Aug;24(15):8480-8490. doi: 10.1111/jcmm.15414. Epub 2020 Jun 27.
The implication of circular RNAs (circRNAs) in the pathogenesis of human cervical cancer (CC) has been demonstrated by numerous of researches, nevertheless, the whole regulatory network of circRNAs in CC remains unclear. In the present study, two GSE data sets (GSE113696 and GSE102686) were enrolled to analysed different expressed circRNA. We found that hsa_circ_0000520(circ_0000520) was decreased in CC tissues and cell lines. Functional studies indicated circ_0000520 overexpression in vitro repressed CC cell proliferation, invasion and migration, while promoted CC cell apoptosis. Moreover, circ_0000520 overexpression in vivo repressed CC tumour growth. Mechanismly, circ_0000520 and PAX5 were revealed to directly bind to miR-146b-3p, and circ_0000520 could indirectly regulate PAX5 by sponging miR-146b-3p. In conclusion, circ_0000520 repressed CC progression in vitro and in vivo by sponging miR-146b-3p to release PAX5.
环状 RNA（circRNAs）在人类宫颈癌（CC）发病机制中的作用已被大量研究证明，但 circRNAs 在 CC 中的整体调控网络仍不清楚。在本研究中，我们纳入了两个 GSE 数据集（GSE113696 和 GSE102686）来分析差异表达的 circRNA。我们发现 hsa_circ_0000520（circ_0000520）在 CC 组织和细胞系中表达降低。功能研究表明，体外过表达 circ_0000520 抑制了 CC 细胞的增殖、侵袭和迁移，同时促进了 CC 细胞的凋亡。此外，体内过表达 circ_0000520 抑制了 CC 肿瘤的生长。机制研究表明，circ_0000520 与 PAX5 直接结合，circ_0000520 可以通过海绵吸附 miR-146b-3p 间接调节 PAX5。总之，circ_0000520 通过海绵吸附 miR-146b-3p 释放 PAX5 来抑制体外和体内 CC 的进展。
