Department of Clinical Skills Experimental Teaching Center, Qiqihar Medical University, Qiqihar 161000, China.
Department of Obstetrics and Gynecology, Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.
Gene. 2019 Jul 15;705:103-108. doi: 10.1016/j.gene.2019.04.068. Epub 2019 Apr 25.
Cervical cancer (CC), a common gynecological carcinoma, is a serious threat to women's health. The dysregulation of circular RNAs (circRNAs) is associated with the pathogenesis of cervical cancer. Therefore, we explored the role of circ-ATP8A2 in CC cell development and progression. Circ-ATP8A2 profiles in CC specimens and cells were detected using real-time PCR. In addition, cell counting kit-8 (CCK-8), acridine orange/ethidium bromide (AO/EB), flow cytometric, and Transwell experiments were carried out on HeLa and SW756 cells to determine cell proliferation, apoptosis, migration and invasion. Furthermore, the mechanism of circ-ATP8A2 was explored by dual-luciferase reporter system. Circ-ATP8A2 was significantly enhanced in CC specimens and cells. Knockdown of circ-ATP8A2 inhibited cell proliferation, migratory and invasive capacities and increased apoptotic cells. Ectopically expressed circ-ATP8A2 induced the opposite effects. For the mechanism exploration, circ-ATP8A2 sponges miR-433 to release its suppression on epidermal growth factor receptor (EGFR) expression at post-transcriptional level. What's more, circ-ATP8A2 could promote cell progression by miR-433/EGFR axis in CC cells. Collectively, this work might offer a potential treatment target for CC. ABBREVIATIONS.
宫颈癌（CC）是一种常见的妇科癌症，严重威胁着妇女的健康。环状 RNA（circRNAs）的失调与宫颈癌的发病机制有关。因此，我们探讨了 circ-ATP8A2 在 CC 细胞发育和进展中的作用。采用实时 PCR 检测 CC 标本和细胞中的 circ-ATP8A2 谱。此外，在 HeLa 和 SW756 细胞上进行细胞计数试剂盒-8（CCK-8）、吖啶橙/溴化乙锭（AO/EB）、流式细胞术和 Transwell 实验，以确定细胞增殖、凋亡、迁移和侵袭。此外，通过双荧光素酶报告系统探讨了 circ-ATP8A2 的机制。CC 标本和细胞中 circ-ATP8A2 显著增强。circ-ATP8A2 敲低抑制细胞增殖、迁移和侵袭能力，增加凋亡细胞。外源性表达 circ-ATP8A2 则诱导相反的效果。为了探索机制，circ-ATP8A2 作为 miR-433 的海绵体，在转录后水平释放对表皮生长因子受体（EGFR）表达的抑制作用。更重要的是，circ-ATP8A2 可以通过 miR-433/EGFR 轴在 CC 细胞中促进细胞进展。总之，这项工作可能为 CC 提供了一个潜在的治疗靶点。缩写词。
