Department of Molecular Diagnostic Pathology, School of Medicine, Iwate Medical University, Iwate, Japan.
Department of Surgery, School of Medicine, Iwate Medical University, Iwate, Japan.
Pathol Int. 2020 Sep;70(9):633-643. doi: 10.1111/pin.12975. Epub 2020 Jun 26.
MicroRNAs (miRNAs) are potential biomarkers of neoplastic lesions, but additional information on dysregulated miRNA expression during progression of the adenoma-adenocarcinoma sequence may be helpful to identify the role of miRNAs in this sequence. We examined the expression levels of 13 miRNAs (hsa-miRNA-19a-3p, hsa-miRNA-21-5p, hsa-miRNA-27a-3p, hsa-miRNA-27b-3p, hsa-miRNA-31-5p, hsa-miRNA-34b-3p, hsa-miRNA-125b-5p, hsa-miRNA-143-3p, miRNA-191-5p, hsa-miRNA-193b-3p, hsa-miRNA-195-5p, hsa-miRNA-206 and hsa-let-7a-5p) that are closely associated with colorectal carcinogenesis in 40 conventional adenomas (tubular and tubulovillous adenomas), 20 intramucosal carcinomas (IMCs) and 60 invasive colorectal cancers (iCRCs) using reverse-transcription polymerase chain reaction. These 120 tumors were divided into two cohorts, that is, cohort 1 (60 cases) and cohort 2 (for validation; 60 cases). We analyzed the expression levels of these miRNAs in the first step (adenoma→IMC) and second step IMC→iCRC) of the adenoma-carcinoma sequence in both cohorts. Although no significant differences in the expression of any of the 13 miRNAs were found between adenomas and IMCs consistently in both cohorts, the expression levels of hsa-miRNA-125b-5p, hsa-miRNA-143-3p, and hsa-miRNA-206 were significantly upregulated in iCRC in both cohorts compared with those in IMC. The current results suggest that certain miRNAs, including hsa-miRNA-125b-5p, hsa-miRNA-143-3p and hsa-miRNA-206, are candidate markers that play critical roles in the progression of IMC to iCRC.
微小 RNA（miRNA）是肿瘤病变的潜在生物标志物，但在腺瘤-腺癌序列进展过程中，miRNA 表达失调的更多信息可能有助于确定 miRNA 在该序列中的作用。我们检查了 13 种 miRNA（hsa-miRNA-19a-3p、hsa-miRNA-21-5p、hsa-miRNA-27a-3p、hsa-miRNA-27b-3p、hsa-miRNA-31-5p、hsa-miRNA-34b-3p、hsa-miRNA-125b-5p、hsa-miRNA-143-3p、miRNA-191-5p、hsa-miRNA-193b-3p、hsa-miRNA-195-5p、hsa-miRNA-206 和 hsa-let-7a-5p）在 40 例常规腺瘤（管状和管状绒毛状腺瘤）、20 例黏膜内癌（IMC）和 60 例浸润性结直肠癌（iCRC）中的表达水平，使用逆转录聚合酶链反应。这 120 个肿瘤分为两个队列，即队列 1（60 例）和队列 2（验证；60 例）。我们在两个队列中分析了这些 miRNA 在腺瘤-癌序列的第一步（腺瘤→IMC）和第二步 IMC→iCRC）中的表达水平。尽管在两个队列中，在腺瘤和 IMC 之间，13 种 miRNA 中的任何一种的表达都没有一致的显著差异，但与 IMC 相比，在两个队列的 iCRC 中，hsa-miRNA-125b-5p、hsa-miRNA-143-3p 和 hsa-miRNA-206 的表达水平显著上调。目前的结果表明，某些 miRNA，包括 hsa-miRNA-125b-5p、hsa-miRNA-143-3p 和 hsa-miRNA-206，是在 IMC 进展为 iCRC 中发挥关键作用的候选标志物。
