Department of Gastroenterology, Jiaozhou Central Hospital of Qingdao, Shandong, China.
Department of Gastroenterology, Yidu Central Hospital of Weifang, Shandong, China.
Bioengineered. 2021 Dec;12(2):10491-10500. doi: 10.1080/21655979.2021.1997694.
An early diagnosis and effective prognostic factors would greatly reduce the mortality rate of colorectal cancer (CRC). This research is intended to complete the evaluation of the prognostic value and potential role of miR-1180-3p in CRC. The miR-1180-3p levels were reduced in CRC patients' tissues, blood, and human CRC cell lines. The ability of miR-1180-3p was explored in discrimination of CRC patients and healths and the value in overall survival estimate. The effect of miR-1180-3p dysregulation on the CRC cellular function was investigated. miR-1180-3p is downregulated in CRC tissues, blood and cells than normal ones. This lower expression was correlated with vascular invasion, lymph node metastasis, and TNM stage. With the use of ROC curve, miR-1180-3p showed discriminating ability in CRC patients and healthy subjects. With the result of Kaplan-Meier analysis and multi-multivariate Cox analysis, miR-1180-3p was an independent predictor for CRC patients' overall survival. Utilizing CCK-8, Transwell and matrigel assays, overexpression of miR-1180-3p reduced cancer cell proliferation and mobility, but induced apoptosis, by targeting . miR-1180-3p might function as a suppressor in CRC progression and allowed the discovery of a new biomarker for diagnosis, prognosis and therapy target for CRC.
早期诊断和有效的预后因素将大大降低结直肠癌（CRC）的死亡率。本研究旨在完成 miR-1180-3p 在 CRC 中的预后价值和潜在作用的评估。CRC 患者组织、血液和人 CRC 细胞系中 miR-1180-3p 水平降低。探讨了 miR-1180-3p 在区分 CRC 患者和健康人群以及估计总生存率方面的价值。研究了 miR-1180-3p 失调对 CRC 细胞功能的影响。miR-1180-3p 在 CRC 组织、血液和细胞中的表达低于正常水平。这种低表达与血管侵犯、淋巴结转移和 TNM 分期有关。通过使用 ROC 曲线，miR-1180-3p 在 CRC 患者和健康受试者中表现出区分能力。通过 Kaplan-Meier 分析和多因素 Cox 分析的结果，miR-1180-3p 是 CRC 患者总生存率的独立预测因子。通过 CCK-8、Transwell 和 matrigel 测定，miR-1180-3p 的过表达通过靶向减少癌细胞的增殖和迁移，但诱导细胞凋亡。miR-1180-3p 可能在 CRC 进展中起抑制作用，并为 CRC 的诊断、预后和治疗靶点提供了新的生物标志物。
