Department of Orthopedics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China.
State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Biotechnol Prog. 2020 Nov;36(6):e3043. doi: 10.1002/btpr.3043. Epub 2020 Aug 18.
Electrospinning is a versatile technology for the fabrication of nanofibrous matrixes to regenerate defects. This study aims to develop a functionalized and electroconductive polymeric matrix to improve rat bone marrow mesenchymal stem cell adhesion, proliferation, and differentiation. Herein, the influence of the chemical composition of the substrate on homogeneous modification of the surface with mussel-inspired polydopamine (PDA) is focused. Accordingly, the deposition of PDA on the surface was proved by Fourier transform infrared spectroscopy. Morphologies of the scaffolds demonstrated homogeneous decoration of the polyvinyl alcohol (PVA)/polyurethane (PU)-polyaniline (PANI) matrixes with PDA, while a lower density of mussel-inspired polymer was observed in bare PU-PANI constructs. Although uniform and dense precipitation of PDA reduced conductivity of scaffolds 1.2 times compared with the samples with a low density of the PDA, 1.1 and 1.2 times enhancement in tensile strength and Young's modulus, respectively, were the strength of the applied process, especially in bone tissue engineering area. Contact angle measurements demonstrated about two times reduction in measured values, which shows improvement in hydrophilicity of PDA-modified PVA/PU-PANI fibers compared with PDA-coated PU-PANI ones. Swelling ratio and mass loss ratio calculations revealed enhancement in measured values as a function of homogeneous and dense coating, which arise from hydrophilicity of the polymeric substrate. The bioactivity test indicated that a dense layer of PDA strongly supports formations of hydroxyapatite-like crystals. Moreover, homogeneous decoration of conductive matrixes with PDA showed suitable cell viability, adhesion, and spreading while cell-scaffolds interactions improved under electrical stimulation. Higher expression of alkaline phosphatase and secretion of Collagen I under the electrical field proved the applicability of modified electroconductive scaffolds for further preclinical and clinical studies to introduce as a reconstructive bone substitute.
静电纺丝是一种用于制造纳米纤维基质以再生缺陷的多功能技术。本研究旨在开发功能化和导电聚合物基质，以提高大鼠骨髓间充质干细胞的黏附、增殖和分化。在此，重点研究了基底的化学成分对贻贝启发的聚多巴胺（PDA）均匀表面修饰的影响。相应地，通过傅里叶变换红外光谱证明了 PDA 在表面上的沉积。支架的形态表明，PVA/PU-PANI 基质表面均匀地用 PDA 进行了修饰，而在裸露的 PU-PANI 结构中观察到贻贝启发聚合物的密度较低。尽管均匀且致密的 PDA 沉淀使支架的电导率降低了 1.2 倍，与低密度 PDA 相比，分别提高了 1.1 倍和 1.2 倍的拉伸强度和杨氏模量，这是施加过程的强度，尤其是在骨组织工程领域。接触角测量表明，测量值降低了约两倍，这表明与 PDA 涂覆的 PU-PANI 相比，PDA 改性的 PVA/PU-PANI 纤维的亲水性得到了改善。溶胀比和质量损失比的计算表明，随着均匀和致密涂层的增加，测量值得到了提高，这是由于聚合物基底的亲水性所致。生物活性测试表明，致密的 PDA 层强烈支持形成类羟基磷灰石晶体。此外，导电基质的均匀装饰 PDA 显示出适宜的细胞活力、黏附和扩展，而在电场下细胞-支架相互作用得到改善。碱性磷酸酶的高表达和 Collagen I 的分泌证明了改性导电支架在进一步的临床前和临床研究中的适用性，可作为一种重建性骨替代物。
